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PMID: 7791781 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional interaction between the POU domain protein Tst-1/Oct-6 and the high-mobility-group protein HMG-I/Y.

Molecular and cellular biology ·Vol. 15 ·No. 7 ·1995-07-00 ·Pages 3738-47

Leger H, Sock E, Renner K, Grummt F, Wegner M

Abstract

The POU domain protein Tst-1/Oct-6 is a transcriptional activator of human papovavirus JC virus in transient transfections. Because of its endogenous expression in myelinating glia, Tst-1/Oct-6 might also be an important determinant for the glia specificity of JC virus in vivo. Activation of viral early and late genes depends on the ability of Tst-1/Oct-6 to interact with an AT-rich element within the viral regulatory region. Here, we show that this element not only is bound by Tst-1/Oct-6 but, in addition, serves as a binding site for the high-mobility-group protein HMG-I/Y. In the presence of HMG-I/Y, Tst-1/Oct-6 exhibited an increased affinity for this AT-rich element. The specificity of this effect was evident from the fact that no stimulation of Tst-1/Oct-6 binding was observed on a site that did not allow binding of HMG-I/Y. In addition, both proteins interacted with each other in solution. Direct contacts were identified between the POU domain of Tst-1/Oct-6 and a short stretch of 10 amino acids in the central portion of HMG-I/Y. These results point to an accessory role for HMG-I/Y in the activation of JC viral gene expression by the POU domain protein Tst-1/Oct-6. In agreement with such a role, HMG-Y synergistically supported the function of Tst-1/Oct-6 in transient transfections, measured on the early promoter of JC virus or on an artificial promoter consisting of only a TATA box and the common binding element for Tst-1 and HMG-I/Y.

MeSH Terms
Base Sequence Chromatin/chemistry DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Viral Genes, Reporter Glioblastoma HMGA1a Protein High Mobility Group Proteins/metabolism Humans JC Virus/genetics Molecular Sequence Data Nerve Tissue Proteins/genetics,metabolism Neuroglia/virology Octamer Transcription Factor-6 POU Domain Factors Promoter Regions, Genetic Protein Binding Transcription Factors/genetics,metabolism Transfection Tumor Cells, Cultured
Chemicals
Chromatin DNA-Binding Proteins High Mobility Group Proteins Nerve Tissue Proteins POU Domain Factors POU3F1 protein, human Transcription Factors HMGA1a Protein Octamer Transcription Factor-6
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leger H
Zentrum für Molekulare Neurobiologie, Universität Hamburg, Germany.
Sock E
Renner K
Grummt F
Wegner M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.7.3738
Published
1995-07-00
Pages
3738-47
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230612
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.15.7.3738 PMC Full Text View journal details
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