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PMID: 2602145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cis-acting sequences from mouse rDNA promote plasmid DNA amplification and persistence in mouse cells: implication of HMG-I in their function.

Nucleic acids research ·Vol. 17 ·No. 23 ·1989-12-11 ·Pages 9909-32

Wegner M, Zastrow G, Klavinius A, Schwender S, Müller F, Luksza H, Hoppe J, Wienberg J, Grummt F

Abstract

Searching for amplification promoting sequences within the murine rDNA cistrons, we isolated two elements from the nontranscribed spacer region. These 370 bp and 423 bp long cis-acting elements, referred to as muNTS1 and muNTS2, are localized 4.1 kb and 4.6 kb upstream the RNA polymerase I transcriptional start site. They contain ca. 50 bp long AT-rich sequences that strongly interact with a protein from nuclear extracts. The protein could be purified and identified as HMG-I. A synthetic oligonucleotide encompassing the AT-rich stretch from muNTS1 is able to substitute for the muNTS elements. A similar sequence from the nontranscribed spacer of rat has previously been reported to be important for the function of the RNA polymerase I enhancer (1). Therefore the interaction of HMG I with the muNTS elements may play a role both in the stimulation of DNA amplification and transcription.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Nucleus/metabolism Cloning, Molecular DNA, Ribosomal/genetics Gene Amplification Genes High Mobility Group Proteins/metabolism L Cells/metabolism Mice Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plasmids Promoter Regions, Genetic RNA Polymerase I/metabolism Restriction Mapping Transcription, Genetic Transfection
Chemicals
DNA, Ribosomal High Mobility Group Proteins Oligonucleotide Probes RNA Polymerase I
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wegner M
Institut für Biochemie, Universität Würzburg, FRG.
Zastrow G
Klavinius A
Schwender S
Müller F
Luksza H
Hoppe J
Wienberg J
Grummt F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.23.9909
Published
1989-12-11
Pages
9909-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335222
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.23.9909 PMC Full Text View journal details
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