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PMID: 1979677 Published · ppublish English Journal Article

The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells.

Nucleic acids research ·Vol. 18 ·No. 24 ·1990-12-25 ·Pages 7357-65

Meijer D, Graus A, Kraay R, Langeveld A, Mulder MP, Grosveld G

Abstract

We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation. Furthermore the gene is transiently up regulated during retinoic acid induced differentiation of P19 EC cells, reaching maximum levels of expression one day after RA addition. Sequence analysis of the cDNA encoding the Oct6 protein indicated that the Oct6 gene is a member of the POU-HOMEO domain gene family. The gene expresses a 3 kb mRNA encoding a 449 amino acid protein with an apparent molecular weight of 45 kD. The sequence of the Oct6 POU domain is identical to that of the rat SCIP (Tst-1) gene. The Oct6 expression pattern suggests a role for this DNA binding protein in neurogenesis as well as early embryogenesis.

Related Genes
Oct6
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Differentiation Cloning, Molecular DNA/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Embryonal Carcinoma Stem Cells Gene Expression Regulation Genes, Homeobox Molecular Sequence Data Molecular Weight Multigene Family Neoplastic Stem Cells Octamer Transcription Factor-6 Transcription Factors/genetics,metabolism Tretinoin/pharmacology
Chemicals
DNA-Binding Proteins Transcription Factors Octamer Transcription Factor-6 Tretinoin DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Meijer D
MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Graus A
Kraay R
Langeveld A
Mulder M P
Grosveld G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.24.7357
Published
1990-12-25
Pages
7357-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332873
Subset
IM
Databases
GENBANK
X54628
External Links
PubMed source DOI: 10.1093/nar/18.24.7357 PMC Full Text View journal details
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