Home LiteratureArticle Details
PMID: 1827203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3720-4

Hai T, Curran T

Abstract

The Fos/Jun and ATF/CREB families of transcription factors function in coupling extracellular signals to alterations in expression of specific target genes. Like many eukaryotic transcription factors, these proteins bind to DNA as dimers. Dimerization is mediated by a structure known as the "leucine-zipper" motif. Although Fos/Jun and ATF/CREB were previously thought to interact preferentially with different DNA regulatory elements (the AP-1/TRE and ATF/CRE sites, respectively), we find that members of these two families form selective cross-family heterodimers. The resulting heterodimers display distinguishable DNA binding specificities from each other and from their parental homodimers. These findings indicate that the Fos/Jun and ATF/CREB families of transcription factors are not as distinct as was previously thought. We suggest that they can be grouped into a superfamily of transcription factors.

Related Genes
ATF-1 ATF-2 ATF-3 ATF-4 c-fos c-jun fra-1
MeSH Terms
Activating Transcription Factor 2 Base Sequence Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins/metabolism,physiology In Vitro Techniques Leucine Zippers Macromolecular Substances Molecular Sequence Data Multigene Family Oligonucleotides/chemistry Precipitin Tests Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Regulatory Sequences, Nucleic Acid Structure-Activity Relationship Transcription Factors/metabolism,physiology
Chemicals
ATF2 protein, human Activating Transcription Factor 2 Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins Macromolecular Substances Oligonucleotides Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hai T
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Curran T
References (46)
46 references, click to expand
  1. A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence.
    Nature. 1989 Feb 23;337(6209):749-52 PMID: 2521922
  2. Viral and cellular fos proteins: a comparative analysis.
    Cell. 1984 Feb;36(2):259-68 PMID: 6319013
  3. Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
    Science. 1989 Mar 31;243(4899):1695-9 PMID: 2494702
  4. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.
    Genes Dev. 1989 Feb;3(2):173-84 PMID: 2497053
  5. Heterodimer formation between CREB and JUN proteins.
    Oncogene. 1990 Mar;5(3):295-302 PMID: 2138276
  6. Direct cloning of leucine zipper proteins: Jun binds cooperatively to the CRE with CRE-BP1.
    Oncogene. 1990 Apr;5(4):451-8 PMID: 2139203
  7. mXBP/CRE-BP2 and c-Jun form a complex which binds to the cyclic AMP, but not to the 12-O-tetradecanoylphorbol-13-acetate, response element.
    Mol Cell Biol. 1990 Apr;10(4):1609-21 PMID: 2138707
  8. The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.
    EMBO J. 1989 Mar;8(3):805-13 PMID: 2498083
  9. Modularity in promoters and enhancers.
    Cell. 1989 Jul 14;58(1):1-4 PMID: 2665940
  10. Purification and characterization of multiple nuclear factors that bind to the TAX-inducible enhancer within the human T-cell leukemia virus type 1 long terminal repeat.
    Mol Cell Biol. 1989 Apr;9(4):1733-45 PMID: 2786141
  11. Evidence that the leucine zipper is a coiled coil.
    Science. 1989 Jan 27;243(4890):538-42 PMID: 2911757
  12. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
    Nature. 1987 Jul 9-15;328(6126):175-8 PMID: 2885756
  13. Viral and cellular fos proteins are complexed with a 39,000-dalton cellular protein.
    Mol Cell Biol. 1985 Jan;5(1):167-72 PMID: 3885008
  14. Isolation and characterization of fra-2, an additional member of the fos gene family.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3619-23 PMID: 2110368
  15. Isolation and characterization of two novel, closely related ATF cDNA clones from HeLa cells.
    Nucleic Acids Res. 1990 Jun 25;18(12):3467-73 PMID: 1694576
  16. Membrane depolarization and calcium induce c-fos transcription via phosphorylation of transcription factor CREB.
    Neuron. 1990 Apr;4(4):571-82 PMID: 2157471
  17. Functional characterization of a cAMP-responsive element of the rat insulin I gene.
    J Biol Chem. 1990 Jan 25;265(3):1465-9 PMID: 2153135
  18. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  19. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  20. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
    Genes Dev. 1989 Dec;3(12B):2083-90 PMID: 2516827
  21. Identification of factors that interact with the E1A-inducible adenovirus E3 promoter.
    Genes Dev. 1987 Dec;1(10):1132-46 PMID: 2828166
  22. Cyclic AMP and the induction of eukaryotic gene transcription.
    J Biol Chem. 1988 Jul 5;263(19):9063-6 PMID: 2837474
  23. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  24. Cyclic AMP and phorbol ester-stimulated transcription mediated by similar DNA elements that bind distinct proteins.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7922-6 PMID: 2847147
  25. EivF, a factor required for transcription of the adenovirus EIV promoter, binds to an element involved in EIa-dependent activation and cAMP induction.
    Genes Dev. 1988 Aug;2(8):975-90 PMID: 2844626
  26. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  27. Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
    Science. 1988 Dec 9;242(4884):1430-3 PMID: 2974179
  28. Purification and functional characterization of a cellular transcription factor that binds to an enhancer element within the adenovirus early EIIa promoter.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2484-8 PMID: 2965812
  29. A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8355-9 PMID: 2960975
  30. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites.
    Science. 1988 Mar 4;239(4844):1150-3 PMID: 2964084
  31. A cellular transcription factor E4F1 interacts with an E1a-inducible enhancer and mediates constitutive enhancer function in vitro.
    EMBO J. 1987 May;6(5):1345-53 PMID: 2956091
  32. Common DNA binding site for Fos protein complexes and transcription factor AP-1.
    Cell. 1988 Feb 12;52(3):471-80 PMID: 3125983
  33. Fos-associated protein p39 is the product of the jun proto-oncogene.
    Science. 1988 May 20;240(4855):1010-6 PMID: 3130660
  34. A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1.
    Genes Dev. 1988 Oct;2(10):1216-26 PMID: 3144478
  35. DNA binding activities of three murine Jun proteins: stimulation by Fos.
    Cell. 1988 Dec 2;55(5):907-15 PMID: 3142691
  36. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro.
    Genes Dev. 1988 Dec;2(12B):1687-99 PMID: 2467839
  37. In vitro transcription directed from the somatostatin promoter is dependent upon a purified 43-kDa DNA-binding protein.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2181-5 PMID: 2564679
  38. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.
    EMBO J. 1989 Jul;8(7):2023-8 PMID: 2529117
  39. Behind the Fos and Jun leucine zipper.
    Cancer Cells. 1989 Nov;1(3):71-6 PMID: 2518283
  40. Identification and purification of a novel 120-kDa protein that recognizes the cAMP-responsive element.
    J Biol Chem. 1990 Feb 25;265(6):3212-8 PMID: 2137455
  41. Characterization of a bipartite activator domain in transcription factor CREB.
    Cell. 1990 Feb 23;60(4):611-7 PMID: 2137373
  42. Analysis of dimerization and DNA binding functions in Fos and Jun by domain-swapping: involvement of residues outside the leucine zipper/basic region.
    Oncogene. 1990 Jun;5(6):929-39 PMID: 2113670
  43. Dimers, leucine zippers and DNA-binding domains.
    Trends Genet. 1990 Feb;6(2):36-40 PMID: 2186528
  44. Transcriptional regulation by dimerization: two sides to an incestuous relationship.
    Cell. 1990 Apr 6;61(1):9-11 PMID: 2180585
  45. A cDNA for a human cyclic AMP response element-binding protein which is distinct from CREB and expressed preferentially in brain.
    Mol Cell Biol. 1990 Apr;10(4):1347-57 PMID: 2320002
  46. Preferential heterodimer formation by isolated leucine zippers from fos and jun.
    Science. 1989 Aug 11;245(4918):646-8 PMID: 2503872
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3720
Published
1991-05-01
Pages
3720-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51524
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.9.3720 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2