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PMID: 1594443 Published · ppublish English Journal Article

Mapping the transactivation domain of the Oct-6 POU transcription factor.

Nucleic acids research ·Vol. 20 ·No. 9 ·1992-05-11 ·Pages 2241-7

Meijer D, Graus A, Grosveld G

Abstract

The POU transcription factor Oct-6 is expressed in embryonic stem cells, glial progenitor cells and in a restricted set of neurons in the CNS. The protein has been shown to act as a transactivator as well as a repressor. Here we show that the Oct-6 protein activates transcription from three different promoters in HeLa cells. The ability to activate a minimal tk promoter via a multimerized IgH enhancer octamer motif relies on a domain within the aminoterminal third of the protein. Parts of this domain can be deleted without abolishing transactivation, suggesting that there is functional redundancy within this region. The transactivation domain of the Oct-6 protein is different from other described activation domains in that it is highly glycine and alanine rich.

MeSH Terms
Animals Base Sequence Binding Sites CHO Cells Chromosome Deletion Cricetinae DNA DNA-Binding Proteins/chemistry,metabolism HeLa Cells Humans Molecular Sequence Data Octamer Transcription Factor-6 Promoter Regions, Genetic Transcription Factors/chemistry,metabolism Transcriptional Activation
Chemicals
DNA-Binding Proteins POU3F1 protein, human Transcription Factors Octamer Transcription Factor-6 DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meijer D
MGC, Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Graus A
Grosveld G
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.9.2241
Published
1992-05-11
Pages
2241-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312337
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.9.2241 PMC Full Text View journal details
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