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PMID: 2808516 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.

The Journal of cell biology ·Vol. 109 ·No. 5 ·1989-11-00 ·Pages 1975-82

Disney JE, Johnson KR, Magnuson NS, Sylvester SR, Reeves R

Abstract

Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.

MeSH Terms
Animals Cell Line Cell Nucleus/ultrastructure Chromosome Banding Chromosomes/ultrastructure Chromosomes, Human/ultrastructure High Mobility Group Proteins/analysis Humans Interphase Leukemia, Erythroblastic, Acute Metaphase Mice Repetitive Sequences, Nucleic Acid Tumor Cells, Cultured/cytology
Chemicals
High Mobility Group Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Disney J E
Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4660.
Johnson K R
Magnuson N S
Sylvester S R
Reeves R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.5.1975
Published
1989-11-00
Pages
1975-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115878
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.109.5.1975 PMC Full Text View journal details
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