Toward a complete linkage map of the human X chromosome: regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids.

American journal of human genetics ·Vol. 36 ·No. 2 ·1984-03-00 ·Pages 265-76

Wieacker P, Davies KE, Cooke HJ, Pearson PL, Williamson R, Bhattacharya S, Zimmer J, Ropers HH

Abstract

Closely linked restriction fragment length polymorphisms (RFLPs) are potentially useful as diagnostic markers of genetic defects, and, in principle, RFLPs can be employed to construct a complete linkage map of the human genome. On the X chromosome, linkage studies are particularly rewarding because in man more than 120 X-linked genes are known. Thus, it is probable that each X-specific RFLP will be of use as a genetic marker of one or several X-linked disorders. To facilitate the search for closely linked RFLPs, we have regionally assigned 16 cloned DNA sequences to various portions of the human X chromosome, employing a large panel of somatic cell hybrids. These probes have been used to correlate genetic and physical distances on Xp, and it can be extrapolated from these data that the number and distribution of available Xq sequences will also suffice to span the long arm of the X chromosome.

MeSH Terms

Animals Cell Line Chromosome Mapping Cloning, Molecular Cricetinae Cricetulus DNA Restriction Enzymes/genetics DNA, Recombinant Electrophoresis, Agar Gel Female Genetic Linkage Humans Hybrid Cells Mice Nucleic Acid Hybridization Polymorphism, Genetic X Chromosome

Chemicals

DNA, Recombinant DNA Restriction Enzymes

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Wieacker P

Davies K E

Cooke H J

Pearson P L

Williamson R

Bhattacharya S

Zimmer J

Ropers H H

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Article Info

Journal

American journal of human genetics

Abbr.

Am J Hum Genet

ISSN

0002-9297

Published

1984-03-00

Pages

265-76

Language

English

Region

United States

NLM ID

0370475

PMCID

PMC1684417

Subset

External Links

PubMed source PMC Full Text View journal details

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Toward a complete linkage map of the human X chromosome: regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids.

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