Home LiteratureArticle Details
PMID: 6952245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1950-4

Brennand J, Chinault AC, Konecki DS, Melton DW, Caskey CT

Abstract

Cloned cDNA sequences of the murine hypoxanthine/guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) gene have been isolated by using a mouse neuroblastoma cell line containing increased levels of a variant HPRT protein. We have used these sequences as probes to demonstrate that protein overproduction in this cell line is a consequence of at least a 20-fold increase in HPRT mRNA levels resulting from approximately 50-fold amplification of HPRT genomic sequences. The largest cDNA insert so far characterized represents about 70% of the HPRT mRNA sequence. This cDNA is shown to possess regions of homology with mRNA and DNA from Chinese hamster, baboon, and human, thus facilitating detailed analysis of this locus in these four species.

MeSH Terms
Animals Cell Line DNA/genetics DNA, Recombinant Gene Amplification Genes Hypoxanthine Phosphoribosyltransferase/genetics Mice Neuroblastoma Plasmids RNA, Messenger/genetics Species Specificity
Chemicals
DNA, Recombinant RNA, Messenger DNA Hypoxanthine Phosphoribosyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brennand J
Chinault A C
Konecki D S
Melton D W
Caskey C T
References (30)
30 references, click to expand
  1. Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity.
    Biochem Genet. 1972 Aug;7(1):33-40 PMID: 5064956
  2. Altered enzymes in drug-resistant variants of mammalian tissue culture cells.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9 PMID: 4522295
  3. Chinese hamster hypoxanthine-guanine phosphoribosyltransferase. Purification, structural, and catalytic properties.
    J Biol Chem. 1974 Jul 10;249(13):4030-7 PMID: 4368495
  4. Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene.
    J Cell Physiol. 1975 Apr;85(2 Pt 1):307-20 PMID: 47331
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Radioimmune determination of hypoxanthine phosphoribosyltransferase crossreacting material in erythrocytes of Lesch-Nyhan patients.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4147-50 PMID: 1060095
  7. Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):926-30 PMID: 265586
  8. The isolation of a suppressible nonsense mutant in mammalian cells.
    Cell. 1977 Oct;12(2):371-81 PMID: 912748
  9. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  10. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  11. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells.
    Cell. 1978 May;14(1):133-41 PMID: 208776
  12. A method for the recovery of DNA from agarose gels.
    Nucleic Acids Res. 1978 Jul;5(7):2321-32 PMID: 673856
  13. Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5553-6 PMID: 281704
  14. The HPRT locus.
    Cell. 1979 Jan;16(1):1-9 PMID: 369702
  15. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.
    Gene. 1979 Feb;5(2):111-26 PMID: 376402
  16. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  17. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  18. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  19. Addition of homopolymers to the 3'-ends of duplex DNA with terminal transferase.
    Methods Enzymol. 1979;68:41-50 PMID: 542126
  20. Localization of loci for hypoxanthine phosphoribosyltransferase and glucose-6-phosphate dehydrogenase and biochemical evidence of nonrandom X chromosome expression from studies of a human X-autosome translocation.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2810-3 PMID: 6930669
  21. Comparative gene mapping: order of loci on the X chromosome is different in mice and humans.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3595-9 PMID: 6251472
  22. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  23. Construction and preliminary characterization of the rat casein and alpha-lactalbumin cDNA clones.
    J Biol Chem. 1981 Jan 10;256(1):526-32 PMID: 7005218
  24. Structural analysis of mutant and revertant forms of Chinese hamster hypoxanthine-guanine phosphoribosyltransferase.
    J Biol Chem. 1981 Mar 25;256(6):2878-86 PMID: 7204379
  25. Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2110-4 PMID: 6941274
  26. Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253-7 PMID: 6166005
  27. Recovery of DNA fragments inserted by the "tailing" method: regeneration of PstI restriction sites.
    Gene. 1981 May;13(4):339-46 PMID: 6266921
  28. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  29. Cell fusion-induced mouse neuroblastomas HPRT revertants with variant enzyme and elevated HPRT protein levels.
    Somatic Cell Genet. 1981 May;7(3):331-44 PMID: 7027497
  30. In vitro translation of hypoxanthine/guanine phosphoribosyltransferase mRNA: characterization of a mouse neuroblastoma cell line that has elevated levels of hypoxanthine/guanine phosphoribosyltransferase protein.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6977-80 PMID: 6947270
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1950
Published
1982-03-00
Pages
1950-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346099
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.6.1950 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2