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PMID: 6461845 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1557-78

Kunkel LM, Tantravahi U, Eisenhard M, Latt SA

Abstract

Fluorescence activated sorting of chromosomes from 49,XXXXY human lymphoblasts has been used to obtain DNA enriched for the human X. This DNA was cloned in lambda phage Charon 21A to obtain a library of approximately 60,000 pfu. Phage inserts free of human highly repeated DNA sequences are localized to different regions of the human X by two independent hybridization analyses. The first utilized comparative hybridization to rodent-human hybrid cell DNA samples containing all or known portions of the human X, while the second was based on hybridization dosage to DNA samples from human cell lines differing in the number of X chromosomes or X chromosome segments. Of five unique sequence inserts tested, three were X chromosome specific and were localized to regions Xpter leads to Xcen, Xql leads to Xq22 and Xq24 leads to Xqter, respectively. The library presented here represents a highly enriched source of human X chromosome-specific DNA sequences.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Cell Line Cloning, Molecular Cricetinae Cricetulus DNA/genetics Female Flow Cytometry Humans Hybrid Cells/physiology Lymphocytes/analysis Nucleic Acid Hybridization Sex Chromosomes/analysis X Chromosome/analysis
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kunkel L M
Tantravahi U
Eisenhard M
Latt S A
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1557
Published
1982-03-11
Pages
1557-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320550
Subset
IM
Grants
NIGMS NIH HHS · GM21121 · United States
NICHD NIH HHS · HD 40807 · United States
External Links
PubMed source DOI: 10.1093/nar/10.5.1557 PMC Full Text View journal details
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