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PMID: 954550 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes.

Chromosoma ·Vol. 57 ·No. 2 ·1976-08-17 ·Pages 135-53

Latt SA, Willard HF, Gerald PS

Abstract

BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolution method for determining the interchange points of X-X and X-autosome translocations. Asynchrony among terminal replication patterns of multiple late replicating X chromosomes within an individual cell can occasionally be demonstrated. The arms of isochromosomes usually exhibit symmetrical fluorescence patterns, with replication terminating in bands Xq21 and Xq23 (predominant pattern) or in bands Xq25 and Xq27 (alternative pattern) in both arms. In the vast majority of lymphocytes containing a balanced X-13 or X-19 translocation, the normal X is late replicating. However, DNA synthesis in the translocation products occasionally appears somewhat delayed relative to that expected for an early replicating X, consistent with possible position effects on replication kinetics.

MeSH Terms
Adolescent Adult Benzimidazoles Bromodeoxyuridine Child Child, Preschool Chromosomes, Human, 13-15 Chromosomes, Human, 19-20 DNA Replication Female Fluorescent Dyes Humans Lymphocytes/cytology Sex Chromosome Aberrations/metabolism Time Factors Translocation, Genetic
Chemicals
Benzimidazoles Fluorescent Dyes Bromodeoxyuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Latt S A
Willard H F
Gerald P S
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53 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292912
Published
1976-08-17
Pages
135-53
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292912 View journal details
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