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PMID: 6324165 Published · ppublish English Journal Article

Structural organization and expression of human DNA sequences related to the transforming gene of avian myeloblastosis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 24 ·1983-12-00 ·Pages 7385-9

Franchini G, Wong-Staal F, Baluda MA, Lengel C, Tronick SR

Abstract

Bacteriophage libraries of human DNA were screened for sequences homologous to the transforming gene (v-myb) of avian myeloblastosis virus. The three overlapping clones isolated were shown to contain a total of 1.0 kilobase pair (kbp) of sequence related to v-myb distributed over 6.2 kbp. Restriction enzyme mapping and heteroduplex analysis revealed the presence of five myb-related domains interrupted by four stretches of non-homology. To study the extent of human DNA coding sequences that constitute the myb gene homologue, c-myb (human), probes spanning about 30 kbp were prepared from the clones and used to study transcription in a human hematopoietic cell line (MOLT-4). Each of the probes hybridized a 4.5-kilobase transcript, which suggests that either the c-myb (human) gene encompasses 30 kbp or it contains two or more transcription units that each give rise to a mRNA of 4.5 kilobases.

MeSH Terms
Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics Base Sequence Cell Transformation, Neoplastic Cloning, Molecular DNA/metabolism DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Humans Liver/metabolism Nucleic Acid Conformation Transcription, Genetic
Chemicals
DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Franchini G
Wong-Staal F
Baluda M A
Lengel C
Tronick S R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.24.7385
Published
1983-12-00
Pages
7385-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389955
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.24.7385 PMC Full Text View journal details
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