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PMID: 6254063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cellular sequences are present in the presumptive avian myeloblastosis virus genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5177-81

Souza LM, Strommer JN, Hillyard RL, Komaromy MC, Baluda MA

Abstract

EcoRI restriction endonuclease fragments from a lambda proviral DNA hybrid containing the entire presumptive avian myeloblastosis virus (AMV) provirus, and from a lambda proviral hybrid containing a partial myeloblastosis-associated virus type 1 (MAV-1)-like provirus were compared by heteroduplex analysis. The cloned presumptive AMV provirus was also analyzed by electron microscopy, using R-loop formation with purified 35S RNA isolated from virions of the standard AMV complex. The results indicate that the putative AMV genome contains a segment absent in its MAV-1-like helper virus. This segment represents a substitution in the region of the genome that in MAV-1 virus is occupied by the envelope gene and is approximately 900 +/- 160 nucleotide pairs in length. Hybridization of specific probes from the presumptive AMV genome to Southern blots of EcoRI-digested cellular DNA has revealed that these substituted sequences are homologous to chicken and duck DNA that is not related to chicken endogenous proviral sequences.

MeSH Terms
Animals Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics Bacteriophage lambda/genetics Base Sequence Chick Embryo Chickens/genetics Cloning, Molecular DNA/genetics DNA, Recombinant Ducks/genetics Escherichia coli/genetics Genes, Synthetic Genetic Vectors Microscopy, Electron Nucleic Acid Hybridization Recombination, Genetic
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Souza L M
Strommer J N
Hillyard R L
Komaromy M C
Baluda M A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5177
Published
1980-09-00
Pages
5177-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350020
Subset
IM
Grants
NCI NIH HHS · CA-09056 · United States
NCI NIH HHS · CA-10197 · United States
NCI NIH HHS · CA-9030 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.9.5177 PMC Full Text View journal details
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