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PMID: 6291031 Published · ppublish English Journal Article

Molecular cloning and chromosomal mapping of a human locus related to the transforming gene of Moloney murine sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5210-4

Prakash K, McBride OW, Swan DC, Devare SG, Tronick SR, Aaronson SA

Abstract

Human DNA was analyzed for the presence of sequences homologous to the transforming gene (v-mos) of Moloney murine sarcoma virus. A single 2.5-kilobase pair (kbp) EcoRI-generated fragment of human DNA was identified by using cloned v-mos as probe. This DNA was molecularly cloned in a bacteriophage vector. By heteroduplex and restriction enzyme analyses, this human DNA fragment, designated c-mos (human), contained a 0.65-kbp region of continuous homology with v-mos and was present as a single copy in human DNA. By testing for the presence of c-mos (human) in somatic cell hybrids possessing various numbers of human chromosomes, as well as in subclones of such hybrids, it was possible to assign c-mos (human) to human chromosome 8.

MeSH Terms
Base Sequence Cell Transformation, Neoplastic Chromosome Mapping Chromosomes, Human/physiology Cloning, Molecular DNA Restriction Enzymes Female Humans Moloney murine leukemia virus/genetics Nucleic Acid Hybridization Placenta Pregnancy
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Prakash K
McBride O W
Swan D C
Devare S G
Tronick S R
Aaronson S A
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5210
Published
1982-09-00
Pages
5210-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346865
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.17.5210 PMC Full Text View journal details
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