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PMID: 6285355 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3637-40

Der CJ, Krontiris TG, Cooper GM

Abstract

Blot hybridization analysis indicated that NIH 3T3 mouse bladder transformed by high molecular weight DNAs of a human bladder and a human lung carcinoma cell line contained new sequences homologous, respectively, to the transforming genes of Harvey (rasH) and Kirsten (rasK) sarcoma viruses. The unique ras sequences were present in multiple independent NIH cell lines transformed in both primary and secondary transfection assays and corresponded to ras sequences normally present in human DNAs. The ras gene product was expressed in NIH cells transformed by bladder carcinoma DNAs and in the human bladder carcinoma cell lines at levels 2- to 4-fold greater than the level observed in nontransformed NIH 3T3 cells. These results indicate that the transforming genes of these human tumor cell lines are the cellular homologs of two retroviral transforming genes.

MeSH Terms
Animals Carcinoma/genetics Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral DNA, Neoplasm/genetics Gene Expression Regulation Genes, Viral Humans Lung Neoplasms/genetics Mice Molecular Weight Neoplasm Proteins/genetics Sarcoma Viruses, Murine/genetics Urinary Bladder Neoplasms/genetics
Chemicals
DNA, Neoplasm Neoplasm Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Der C J
Krontiris T G
Cooper G M
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3637
Published
1982-06-00
Pages
3637-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346478
Subset
IM
Grants
NCI NIH HHS · CA18689 · United States
NCI NIH HHS · CA28946 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.11.3637 PMC Full Text View journal details
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