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PMID: 6940134 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming activity of human tumor DNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 1181-4

Krontiris TG, Cooper GM

Abstract

High molecular weight DNAs of 26 human tumors and tumor cell lines were assayed for the presence of transmissible activated transforming genes by transfection of NIH 3T3 mouse cells. DNAs of two bladder carcinoma cell lines induced transformation with high efficiencies (approximately 0.2 transformant per microgram of DNA), whereas DNAs of the other tumors studied lacked detectable transforming activity. These findings suggest that dominant mutations or gene rearrangements can result in the activation of cellular transforming genes in some human tumors.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cells, Cultured DNA, Neoplasm/genetics DNA, Viral/biosynthesis Female Humans Mice Nucleic Acid Hybridization Transfection Urinary Bladder Neoplasms/genetics
Chemicals
DNA, Neoplasm DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krontiris T G
Cooper G M
References (13)
13 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.1181
Published
1981-02-00
Pages
1181-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319971
Subset
IM
Grants
NCI NIH HHS · CA18689 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.2.1181 PMC Full Text View journal details
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