Home LiteratureArticle Details
PMID: 6253666 Published · ppublish English Journal Article

Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus.

Journal of virology ·Vol. 36 ·No. 2 ·1980-11-00 ·Pages 408-20

Ellis RW, DeFeo D, Maryak JM, Young HA, Shih TY, Chang EH, Lowy DR, Scolnick EM

Abstract

Detailed restriction endonuclease maps were developed for Harvey murine sarcoma virus (Ha-MuSV) DNA (clone H-1), molecularly closed at its unique EcoRI site in pBR322, for three nonoverlapping subgenomic HindIII clones which together span the entire H-1 clone and for a molecularly cloned DNA copy of a portion of rat 30S RNA (which represents the majority of the rat genetic sequences in Ha-MuSV). Molecular hybridization of the 30S clone to small restriction fragments of clone H-1 revealed a 0.9-to-1.0-kilobase pair region in the 5' half of the Ha-MuSV genome not homologous to the 30S clone, although the 30S clone did contain related sequences in Ha-MuSV on both sides of this nonhomologous region. By using cloned sequences from a segment of the Ha-MuSV nonhomology region as a probe for hybridization to Southern blots of DNA from rat, mouse, bat, and chicken cells, one to three bands were detected in DNA of each species. By contrast, the 30S clone DNA was highly related to many sequences in rat DNA, partially related to fewer mouse DNA sequences, and homologous only to one to three bands in bat and chicken DNA. Earlier work had shown that the 5' half of the Ha-MuSV genome coded for transformation and for the viral p21 protein (Chang et al., J. Virol. 35: 76--92, 1980; Wei et al., Proc. Natl. Acad. Sci. U.S.A., in press). We used two subgenomic HindIII clones whose shared HindIII site mapped within the 5' region of clone H-1 nonhomologous to the 30S clone to test whether the nonhomologous segment might encode the transforming and p21 functions. Although neither of the subgenomic HindIII fragments by themselves induced transformation, ligation of these two nontransforming DNAs to each other did restore p21-mediated transformation. A conclusion consistent with these results is that a region in the 5' half of the Ha-MuSV genome evolutionarily distinct from and not present in rat 30S RNA is essential for transformation and for p21 encoding.

MeSH Terms
Animals Base Sequence Biological Evolution Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Chickens/genetics Chiroptera/genetics Cloning, Molecular DNA, Viral/genetics Genes, Viral Mice/genetics Rats/genetics Sarcoma Viruses, Murine/genetics
Chemicals
DNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ellis R W
DeFeo D
Maryak J M
Young H A
Shih T Y
Chang E H
Lowy D R
Scolnick E M
References (39)
39 references, click to expand
  1. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
    J Virol. 1969 Nov;4(5):549-53 PMID: 4311790
  3. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  4. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  5. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  6. Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information.
    J Virol. 1974 Jun;13(6):1211-9 PMID: 4364897
  7. Sarcoma-virus-related RNA sequences in normal rat cells.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4503-7 PMID: 4140505
  8. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end.
    J Virol. 1975 Oct;16(4):1051-70 PMID: 170411
  9. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  10. Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4650-4 PMID: 172913
  11. Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses.
    J Mol Biol. 1976 Mar 5;101(3):349-65 PMID: 176368
  12. Fractionation of DNA nucleotide transcripts from Moloney sarcoma virus and isolation of sarcoma virus-specific complementary DNA.
    J Virol. 1976 May;18(2):481-90 PMID: 178890
  13. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  14. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
    Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
  15. Structure of the genome of Moloney murine leukemia virus: a terminally redundant sequence.
    Cell. 1978 Apr;13(4):761-73 PMID: 657274
  16. Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.
    J Virol. 1978 May;26(2):291-8 PMID: 26810
  17. In vitro isolation of stable rat sarcoma viruses.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2972-6 PMID: 208081
  18. Comparison of the genomic organization of Kirsten and Harvey sarcoma viruses.
    J Virol. 1978 Jul;27(1):45-55 PMID: 211254
  19. Nucleotide sequences related to the transforming gene of avian sarcoma virus are present in DNA of uninfected vertebrates.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4102-6 PMID: 212733
  20. Expression of RNA of an endogenous replication-defective retrovirus in rat mammary adenocarcinomas induced by 7,12-dimethylbenz[a]anthracene.
    J Natl Cancer Inst. 1978 Nov;61(5):1329-37 PMID: 213614
  21. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.
    Eur J Biochem. 1978 Nov 2;91(1):303-10 PMID: 363426
  22. Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus.
    J Virol. 1979 Mar;29(3):964-72 PMID: 221684
  23. In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus.
    Cell. 1979 Apr;16(4):863-74 PMID: 88264
  24. Identification of a sarcoma virus-coded phosphoprotein in nonproducer cells transformed by Kirsten or Harvey murine sarcoma virus.
    Virology. 1979 Jul 15;96(1):64-79 PMID: 223311
  25. Avian reticuloendotheliosis viruses: evolutionary linkage with mammalian type C retroviruses.
    J Virol. 1979 May;30(2):508-14 PMID: 89204
  26. Inhibition of spontaneous transformation of rat embryo cells releasing endogenous type C virus by virus-specific antiserum.
    J Natl Cancer Inst. 1979 Sep;63(3):745-50 PMID: 89211
  27. p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation.
    J Virol. 1979 Aug;31(2):546-6 PMID: 225570
  28. Three new types of viral oncogene of cellular origin specific for haematopoietic cell transformation.
    Nature. 1979 Oct 11;281(5731):452-5 PMID: 226888
  29. Different rat-derived transforming retroviruses code for an immunologically related intracellular phosphoprotein.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3523-7 PMID: 226970
  30. Heterogeneity of genetic loci in chickens: analysis of endogenous viral and nonviral genes by cleavage of DNA with restriction endonucleases.
    Cell. 1979 Oct;18(2):347-59 PMID: 227605
  31. Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9 PMID: 228288
  32. Heteroduplex analysis of the sequence relationships between the genomes of Kirsten and Harvey sarcoma viruses, their respective parental murine leukemia viruses, and the rat endogenous 30S RNA.
    J Virol. 1979 Sep;31(3):752-60 PMID: 229250
  33. Molecular cloning of the Harvey sarcoma virus closed circular DNA intermediates: initial structural and biological characterization.
    J Virol. 1979 Sep;31(3):795-809 PMID: 229252
  34. Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences.
    J Virol. 1979 Dec;32(3):860-75 PMID: 229270
  35. Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6314-8 PMID: 392518
  36. Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus.
    Science. 1980 Mar 14;207(4436):1222-4 PMID: 6243788
  37. Molecular cloning of the Harvey sarcoma virus circular DNA intermediates. II. Further structural analyses.
    J Virol. 1980 Feb;33(2):845-55 PMID: 6251253
  38. Functional organization of the Harvey murine sarcoma virus genome.
    J Virol. 1980 Jul;35(1):76-92 PMID: 6251279
  39. AN UNIDENTIFIED VIRUS WHICH CAUSES THE RAPID PRODUCTION OF TUMOURS IN MICE.
    Nature. 1964 Dec 12;204:1104-5 PMID: 14243400
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.36.2.408-420.1980
Published
1980-11-00
Pages
408-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353657
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.36.2.408-420.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2