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PMID: 172913 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4650-4

Scolnick EM, Howk RS, Anisowicz A, Peebles PT, Scher CD, Parks WP

Abstract

We have studied the nucleic acid sequences in nonproducer cells transformed by Moloney sarcoma virus or Abelson leukemia virus (two types of replication-defective, RNA-containing, viruses isolated by passage of Moloney leukemia virus in BALB/c mice). DNA probes from the Moloney leukemia in virus detect RNA in both Abelson virus-transformed nonproducer cells and Moloney sarcoma virus-transformed nonproducer cells. A sarcoma-specific cDNA, prepared from the Moloney sarcoma virus, has extensive homology to RNA found in heterologous nonproducer cells transformed by Moloney sarcoma virus, has little homology to RNA in cells producing Moloney leukemia virus, and no detectable homology to RNA in nonproducer cells transformed by the Abelson virus. By analogy to earlier data on avian and mammalian sarcoma viruses, these results suggest that the Moloney sarcoma virus arose by recombination between a portion of the Moloney leukemia virus genome and additional sarcoma-specific information, and indicate that the expression of this information in not essential for Abelson virus-mediated fibroblast transformation.

MeSH Terms
Cell Line Cell Transformation, Neoplastic/pathology Gammaretrovirus/analysis Leukemia Virus, Murine/analysis,pathogenicity Moloney murine leukemia virus/analysis,pathogenicity Nucleic Acid Hybridization RNA, Viral/analysis Sarcoma Viruses, Murine/analysis,pathogenicity
Chemicals
RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Scolnick E M
Howk R S
Anisowicz A
Peebles P T
Scher C D
Parks W P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4650
Published
1975-11-00
Pages
4650-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388781
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4650 PMC Full Text View journal details
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