Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 7 ·1971-07-00 ·Pages 1520-4

Bassin RH, Phillips LA, Kramer MJ, Haapala DK, Peebles PT, Nomura S, Fischinger PJ

Abstract

Small numbers of virus-like particles were observed by electron microscopy in each of two cloned lines of 3T3 cells transformed by murine sarcoma virus, even though these lines were free of detectable quantities of infectious leukemia and sarcoma virus. The morphology and occurrence of the particles were identical to those of the murine leukemia-sarcoma group. Moreover, the particles incorporated uridine and had a buoyant density of 1.16 g/ml in sucrose gradients. No evidence of sarcoma or leukemia virus infectivity was associated with the particles in cells of several susceptible species under various conditions, including both cosedimentation with leukemia virus and infection in the presence of inactivated Sendai virus. The particles may represent a form of murine sarcoma virus deficient in one or more of the viral components necessary for infectivity.

MeSH Terms

Animals Cats Cell Line Centrifugation, Density Gradient Culture Techniques Gammaretrovirus Helper Viruses Humans Kidney Leukemia Virus, Murine Mice Microscopy, Electron Moloney murine leukemia virus Parainfluenza Virus 1, Human Rats Transformation, Genetic Tritium Ultracentrifugation Uridine Virus Replication

Chemicals

Tritium Uridine

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Bassin R H

Phillips L A

Kramer M J

Haapala D K

Peebles P T

Nomura S

Fischinger P J

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.7.1520

Published

1971-07-00

Pages

1520-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389231

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.7.1520 PMC Full Text View journal details

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Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus.

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