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PMID: 6302322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction and isolation of a transforming murine retrovirus containing the src gene of Rous sarcoma virus.

Journal of virology ·Vol. 46 ·No. 2 ·1983-05-00 ·Pages 594-605

Anderson SM, Scolnick EM

Abstract

Recombinant murine retroviruses containing the src gene of the avian retrovirus Rous sarcoma virus were isolated. Such viruses were isolated from cells after transfection with DNAs in which the src gene was inserted into the genome of the amphotropic murine retrovirus 4070A. The isolated viruses had functional gag and pol genes, but they were all env defective since the src gene was inserted in the middle of the env gene coding region. Infectious transforming virus could be isolated only from cells transfected with DNA constructions in which the src gene was in the same polarity as that of a long terminal repeat of the amphotropic viral genome. These recombinant viruses encoded a pp60src protein with a molecular weight similar to that of the Schmidt-Ruppin strain of Rous sarcoma virus. In addition, the src protein(s) of these recombinant viruses was as active as protein kinases in the immune complex protein kinase assay. Intravenous injection of helper-independent Moloney and Friend murine leukemia virus pseudotypes of the src recombinant viruses into 6-week-old NIH Swiss mice resulted in the appearance of splenic foci within 2 weeks, splenomegaly and, later after infection (8 to 10 weeks), anemia. Infectious transforming virus could be recovered from the spleens of diseased animals. Such viruses encoded pp60src but not p21ras or mink cell focus-forming virus-related glycoproteins.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cell Line Cell Transformation, Viral Cloning, Molecular Gene Products, gag Genes, Viral Mice Neoplasms, Experimental/etiology Oncogene Protein pp60(v-src) Protein Kinases/metabolism Retroviridae/genetics,physiology Transfection Viral Envelope Proteins Viral Proteins/genetics
Chemicals
Gene Products, gag Viral Envelope Proteins Viral Proteins Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anderson S M
Scolnick E M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.2.594-605.1983
Published
1983-05-00
Pages
594-605
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255162
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.46.2.594-605.1983 PMC Full Text View journal details
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