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PMID: 6264165 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Analysis of the myeloproliferative sarcoma virus genome: limited changes in the prototype lead to altered target cell specificity.

Journal of virology ·Vol. 38 ·No. 3 ·1981-06-00 ·Pages 952-7

Pragnell IB, Fusco A, Arbuthnott C, Smadja-Joffe F, Klein B, Jasmin C, Ostertag W

Abstract

The myeloproliferative sarcoma virus (MPSV) derived from Moloney sarcoma virus (MSV-Mol) is a unique sarcoma virus which causes expansion of the hematopoietic stem cell compartment as well as the erythroid and myeloid cell lineages. MPSV also induces spleen focus formation in adult mice as do Friend and Rauscher viruses. Analysis of the MPSV genome on methyl mercury gels showed that the genome size is 7.0 kilobases, which is larger than the defective genome of any known MSV-Mol isolate. Hybridization analysis with specific cDNA probes showed that MPSV is a modified sarcoma virus with no sequences in the unique region of the defective sarcoma genome related to unique Friend virus sequences. The only viral sequences in the defective genome other than helper virus-related sequences are derived from the Moloney sarcoma virus genome with no new cellular sequences added. There was no evidence for induction of xenotropic virus sequences in MPSV-infected spleens of DBA/2J mice, indicating that spleen focus formation can be obtained by different mechanisms.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral DNA Genes, Viral Kirsten murine sarcoma virus/genetics Mice Mice, Inbred DBA Nucleic Acid Hybridization RNA, Viral/genetics Sarcoma Viruses, Murine/genetics Spleen/microbiology
Chemicals
RNA, Viral DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pragnell I B
Fusco A
Arbuthnott C
Smadja-Joffe F
Klein B
Jasmin C
Ostertag W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.3.952-957.1981
Published
1981-06-00
Pages
952-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171233
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.38.3.952-957.1981 PMC Full Text View journal details
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