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PMID: 6283105 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence the pp60src, the product of the Rous sarcoma virus src gene, undergoes autophosphorylation.

Journal of virology ·Vol. 41 ·No. 1 ·1982-01-00 ·Pages 1-7

Purchio AF

Abstract

pp60src, the product of the rous sarcoma virus src gene, was purified greater than 100,000-fold by a combination of ion-exchange and immunoaffinity chromatography. Incubation of pp60src purified in this fashion with [32P-gamma]ATP resulted in a single 32P-labeled protein when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Staining of these gels with silver nitrate showed a predominant 60,000-dalton polypeptide which comigrated with the protein labeled with 32P in vitro. Partial digestion of this protein with V8 protease after in vitro iodination indicated that it was pp60src. These results suggest that pp60src is able to autophosphorylate.

MeSH Terms
Avian Sarcoma Viruses/genetics Electrophoresis, Polyacrylamide Gel Genes, Viral Oncogene Protein pp60(v-src) Phosphorylation Viral Proteins/genetics,isolation & purification,metabolism
Chemicals
Viral Proteins Oncogene Protein pp60(v-src)
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Purchio A F
References (19)
19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.41.1.1-7.1982
Published
1982-01-00
Pages
1-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256720
Subset
IM
Grants
NCI NIH HHS · CA 15823 · United States
NCI NIH HHS · CA 21117 · United States
External Links
PubMed source DOI: 10.1128/JVI.41.1.1-7.1982 PMC Full Text View journal details
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