RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 4 ·1970-12-00 ·Pages 1789-96

Scolnick E, Rands E, Aaronson SA, Todaro GJ

Abstract

The RNA-dependent DNA polymerase(s) in murine leukemia, murine mammary tumor, and avian myeloblastosis viruses require maganese for optimal activity. The transcription of added synthetic polyribonucleotides is greatly enhanced when manganese is used in place of magnesium. A soluble RNA-dependent DNA polymerase activity has been released from murine leukemia particles in the presence of manganese and high detergent concentrations. Two RNA viruses, visna virus of sheep and a primate syncytial virus, not known to have tumor-producing ability, also contain the polymerase.

MeSH Terms

Adenine Nucleotides/metabolism Animals Avian Leukosis Virus/enzymology DNA Nucleotidyltransferases/metabolism DNA, Viral/biosynthesis Guanine Nucleotides/metabolism Magnesium/pharmacology Mammary Tumor Virus, Mouse/enzymology Manganese/pharmacology Mice Nucleotides/metabolism Polynucleotides/pharmacology Prions/enzymology RNA Viruses/enzymology RNA, Viral Rauscher Virus/enzymology Stimulation, Chemical Templates, Genetic Tritium

Chemicals

Adenine Nucleotides DNA, Viral Guanine Nucleotides Nucleotides Polynucleotides Prions RNA, Viral Tritium Manganese DNA Nucleotidyltransferases Magnesium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Scolnick E

Rands E

Aaronson S A

Todaro G J

References (12)

12 references, click to expand

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RNA polymerase activity in purified reoviruses.
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Nucleic acid content of visna virus.
Proc Soc Exp Biol Med. 1969 Jul;131(3):927-33 PMID: 4978246
Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.
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RNA-dependent DNA polymerase in virions of RNA tumour viruses.
Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion.
Proc Natl Acad Sci U S A. 1970 Jun;66(2):572-6 PMID: 4317920
Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.
Nature. 1970 Oct 31;228(5270):430-2 PMID: 4320564
DNA polymerases of Rous sarcoma virus: delineation of two reactions with actinomycin.
Nature. 1970 Oct 31;228(5270):433-5 PMID: 4320565
Separation of RNA-dependent DNA polymerase activity from the murine leukaemia virion.
Nature. 1970 Oct 31;228(5270):435-8 PMID: 4320566
ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. III. THE RIBONUCLEIC ACID-PRIMED SYNTHESIS OF RIBONUCLEIC ACID WITH MICROCOCCUS LYSODEIKTICUS RIBONUCLEIC ACID POLYMERASE.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.4.1789

Published

1970-12-00

Pages

1789-96

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283428

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.4.1789 PMC Full Text View journal details

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RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.

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