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PMID: 6300838 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 2 ·1983-01-00 ·Pages 383-7

Shimizu K, Goldfarb M, Perucho M, Wigler M

Abstract

DNA from the human neuroblastoma cell line SK-N-SH is capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. Using genetic selection with the Escherichia coli sup F gene, we have isolated human sequences from mouse cells responsible for the oncogenic transformation. These sequences are present in all human DNAs surveyed and no gross rearrangements of these sequences are found in SK-N-SH cells. Although clearly distinct from two other human transforming genes present in bladder, lung, and colon carcinoma cell lines, all three transforming gene sequences may be related members of the ras gene family.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Cell Line Cell Transformation, Neoplastic Cells, Cultured DNA Restriction Enzymes DNA, Neoplasm/genetics,isolation & purification Humans Mice Neuroblastoma/genetics Nucleic Acid Hybridization Oncogenes Plasmids
Chemicals
DNA, Neoplasm DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shimizu K
Goldfarb M
Perucho M
Wigler M
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.2.383
Published
1983-01-00
Pages
383-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393381
Subset
IM
Grants
NCI NIH HHS · CA 29569 · United States
NIGMS NIH HHS · GM 27643 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.2.383 PMC Full Text View journal details
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