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PMID: 7063039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.

Nature ·Vol. 296 ·No. 5856 ·1982-04-01 ·Pages 404-9

Goldfarb M, Shimizu K, Perucho M, Wigler M

Abstract

DNA from T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells. Using techniques of gene rescue to clone the gene responsible for this transformation, we have found that it is human in origin, less than 5 kilobase pairs in size and is homologous to a 1,100-base polyadenylated RNA species found in T24 and HeLa cells. Blot analysis indicates extensive restriction endonuclease polymorphism near this gene, in human DNAs.

MeSH Terms

Animals Cell Line Cell Transformation, Neoplastic/pathology Cloning, Molecular/methods DNA, Neoplasm/genetics Female Gene Expression Regulation Genes Genetic Linkage Humans Mice Nucleic Acid Hybridization Placenta/physiology Polymorphism, Genetic Pregnancy Transformation, Genetic Urinary Bladder Neoplasms/genetics

Chemicals

DNA, Neoplasm

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Goldfarb M

Shimizu K

Perucho M

Wigler M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/296404a0

Published

1982-04-01

Pages

404-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/296404a0 View journal details

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Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.

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