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PMID: 3453109 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Structure and expression of the Chinese hamster thymidine kinase gene.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 1998-2010

Lewis JA

Abstract

My colleagues and I have cloned a nearly full-length Chinese hamster thymidine kinase (TK) cDNA in a lambda gt10 vector and characterized this cDNA by nucleotide sequencing. The hamster TK protein is encoded in this cDNA by a 702-base-pair open reading frame which specifies a 25,625-dalton protein closely homologous to the previously described human and chicken TK proteins. Using cDNA nucleotide sequence data in conjunction with sequence data derived from selected subclones of the hamster TK gene recombinant phage lambda HaTK.5, we have resolved the structure of the TK gene, finding the 1,219 base pairs of the cDNA sequence to be distributed through 11.2 kilobases of genomic DNA in at least seven exon segments. In addition, we have constructed a variety of Chinese hamster TK minigenes and exonuclease III-S1 derivatives of these genes which have permitted us to define the limits of the Chinese hamster TK gene promoter and demonstrate that efficient TK transformation of Ltk- cells by TK minigenes depends on the presence of both TK intervening sequences and sequences 3' to the site of mRNA polyadenylation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cricetinae/genetics Cricetulus/genetics DNA/genetics Exons Gene Expression Regulation Genes Introns Molecular Weight Promoter Regions, Genetic Thymidine Kinase/genetics
Chemicals
DNA Thymidine Kinase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lewis J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.1998-2010.1986
Published
1986-06-00
Pages
1998-2010
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367739
Subset
IM
Databases
GENBANK
L00364, L00365, L00366, L00367, L00368, L00369, M13622, N00034
External Links
PubMed source DOI: 10.1128/mcb.6.6.1998-2010.1986 PMC Full Text View journal details
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