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PMID: 6092937 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic and physical analysis of the chicken tk gene.

Molecular and cellular biology ·Vol. 4 ·No. 9 ·1984-09-00 ·Pages 1769-76

Merrill GF, Harland RM, Groudine M, McKnight SL

Abstract

Several aspects of the structure of the chicken thymidine kinase gene (tk) have been resolved as a result of genetic experiments and nucleotide sequencing. Deletion mapping established the locations of two functional boundaries in a region thought to correspond to the 5' terminus of the gene. One such boundary coincides with a transcriptional promoter, and the other coincides with the translation start codon of the chicken tk polypeptide. Similar deletion mapping assays identified a functional boundary at the 3' terminus of the gene. DNA sequence analysis confirms the prediction that this 3' region encodes the carboxyl terminus of the tk polypeptide. A recombinant cDNA clone complementary to genomic tk sequences was isolated. A comparison between genomic and cDNA sequences reveals the locations of six intervening sequences and allows prediction of the complete amino acid sequence of the chicken tk polypeptide.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Chickens Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes L Cells/enzymology Mice Nucleic Acid Hybridization Thymidine Kinase/genetics

Chemicals

DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Merrill G F

Harland R M

Groudine M

McKnight S L

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24 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.4.9.1769-1776.1984

Published

1984-09-00

Pages

1769-76

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC368985

Subset

Grants

NIA NIH HHS · AG 03180 · United States

Databases

GENBANK

K02611

External Links

PubMed source DOI: 10.1128/mcb.4.9.1769-1776.1984 PMC Full Text View journal details

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