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PMID: 6656761 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic effects of chromosomal rearrangements in Chinese hamster ovary cells: expression and chromosomal assignment of TK, GALK, ACP1, ADA, and ITPA loci.

Molecular and cellular biology ·Vol. 3 ·No. 11 ·1983-11-00 ·Pages 1967-74

Stallings RL, Adair GM, Siciliano J, Greenspan J, Siciliano MJ

Abstract

Polyethylene glycol-mediated fusion of Chinese hamster ovary (CHO) cells with mouse Cl1D cells produced interspecific somatic cell hybrids which slowly segregated CHO chromosomes. Cytogenetic and isozyme analysis of HAT- and bromodeoxyuridine-selected hybrid subclones and of members of a hybrid clone panel retaining different combinations of CHO chromosomes enabled provisional assignments of the following enzyme loci to CHO chromosomes: TK, GALK, and ACP1 to chromosome 7; TK and GALK to chromosome Z13; ACP1, ADA, and ITPA to chromosome Z8; and ADA and ITPA to chromosome Z9. These genetic markers reflect the origin of each of these Z group chromosomes and indicate the functional activity of alleles located on rearranged chromosomes. Identification of diploid electrophoretic shift mutations for ADA and ITPA was consistent with those observations. Assignment of the functional TK locus in TK+/- CHO-AT3-2 cells indicated that gene deletion may be responsible for TK hemizygosity in this subline.

MeSH Terms
Alleles Animals Cell Line Chromosome Mapping Chromosomes Cricetinae/genetics Cricetulus/genetics Enzymes/genetics Female Genes Hybrid Cells/enzymology Mice Ovary Translocation, Genetic
Chemicals
Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stallings R L
Adair G M
Siciliano J
Greenspan J
Siciliano M J
References (26)
26 references, click to expand
  1. A rapid banding technique for human chromosomes.
    Lancet. 1971 Oct 30;2(7731):971-2 PMID: 4107917
  2. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.
    Chromosoma. 1973;41(2):129-44 PMID: 4120885
  3. On the nature of hereditable variation in cultured somatic cells.
    Cell. 1976 Jan;7(1):1-11 PMID: 779953
  4. Proposed banding nomenclature for the Chinese hamster chromosomes (Cricetulus griseus).
    Cytogenet Cell Genet. 1976;16(1-5):83-91 PMID: 975930
  5. Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration.
    Somatic Cell Genet. 1976 May;2(3):271-80 PMID: 1028174
  6. Chromosome loss is responsible for segregation at the HPRT locus in Chinese hamster cell hybrids.
    Somatic Cell Genet. 1977 Sep;3(5):539-51 PMID: 562539
  7. Chromosome stability in CHO cells.
    Somatic Cell Genet. 1977 Jan;3(1):27-45 PMID: 601677
  8. Electrophoretic shift mutants in Chinese hamster ovary cells: evidence for genetic diploidy.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1919-23 PMID: 273919
  9. Evidence for variation in the number of functional gene copies at the AmaR locus in Chinese hamster cell lines.
    J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):461-7 PMID: 730780
  10. International system for human gene nomenclature (1979) ISGN (1979).
    Cytogenet Cell Genet. 1979;25(1-4):96-116 PMID: 295268
  11. The Chinese hamster gene map. Assignment of four genes (DTS, PGM2, 6PGD, EnoI) to chromosome 2.
    Exp Cell Res. 1980 May;127(1):47-54 PMID: 6445830
  12. Linkage of genetic markers emt and chr in Chinese hamster cells.
    Somatic Cell Genet. 1980 Mar;6(2):215-224 PMID: 6930704
  13. Mutagenicity testing in mammalian cells. I. Derivation of a Chinese hamster ovary cell line heterozygous for the adenine phosphoribosyltransferase and thymidine kinase loci.
    Mutat Res. 1980 Sep;72(2):187-205 PMID: 6449663
  14. Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes.
    J Cell Biol. 1980 Dec;87(3 Pt 1):697-702 PMID: 7193212
  15. Genetic polymorphisms and gene expression variations at enzyme loci in chinese hamster cell lines.
    Somatic Cell Genet. 1981 May;7(3):295-306 PMID: 7197402
  16. Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 7.
    Somatic Cell Genet. 1981 Nov;7(6):683-98 PMID: 7323947
  17. Transfer of genes to Chinese hamster ovary cells by DNA-mediated transformation.
    Somatic Cell Genet. 1982 Jan;8(1):23-39 PMID: 6285530
  18. Structural and functional hemi- and dizygous Chinese hamster chromosome 2 gene loci in CHO cells.
    Somatic Cell Genet. 1982 Jul;8(4):413-22 PMID: 7123449
  19. Assignment of ADA, ITPA, AK1, and AK2 to Chinese hamster chromosomes. Genetic and structural evidence for the conservation of mammalian autosomal synteny.
    J Hered. 1982 Nov-Dec;73(6):399-404 PMID: 7153494
  20. Provisional assignment of TPI, GPI, and PEPD to Chinese hamster autosomes 8 and 9: a cytogenetic basis for functional haploidy of an autosomal linkage group in CHO cells.
    Cytogenet Cell Genet. 1983;35(1):15-20 PMID: 6825466
  21. Electrophoretically detectable mutations induced in CHO cells by varying doses of ultraviolet radiation.
    Mutat Res. 1983 Jan;107(1):167-76 PMID: 6828036
  22. Genetic control of drug resistance: assignment of ama-1 to Chinese hamster chromosome 7, confirmation of assignment of genes coding for TK, GALK, and ACP to chromosome 7, and tentative assignment of TPI to chromosome 8.
    Somatic Cell Genet. 1983 Mar;9(2):235-48 PMID: 6836456
  23. Gene mapping and linkage analysis in Chinese hamster: assignment of the genes for APRT, LDHA, IDH2, and GAA to chromosome 3.
    Somatic Cell Genet. 1983 Jul;9(4):477-87 PMID: 6353627
  24. Evolutionary conservatism in the arrangement of autosomal gene loci in mammals.
    Isozymes Curr Top Biol Med Res. 1983;10:313-21 PMID: 6354987
  25. EFFECT OF HALOGENATED PYRIMIDINES AND THYMIDINE ON GROWTH OF L-CELLS AND A SUBLINE LACKING THYMIDINE KINASE.
    Exp Cell Res. 1964 Jan;33:19-28 PMID: 14109133
  26. THE USE OF GENETIC VARIATION IN THE ANALYSIS OF ISOZYME STRUCTURE.
    Brookhaven Symp Biol. 1964 Dec;17:117-30 PMID: 14246256
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.11.1967-1974.1983
Published
1983-11-00
Pages
1967-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC370064
Subset
IM
Grants
NCI NIH HHS · CA-04484 · United States
NCI NIH HHS · CA-28711 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.11.1967-1974.1983 PMC Full Text View journal details
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