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PMID: 6959133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 21 ·1982-11-00 ·Pages 6522-6

Gasser CS, Simonsen CC, Schilling JW, Schimke RT

Abstract

We have constructed a number of abbreviated dihydrofolate reductase (DHFR) genes by using cloned mouse genomic and cDNA sequences. These genes contain 1.0 kilobase of 5' flanking genomic sequence and varying portions of the 3' non-coding region. Two of the genes contain the first two introns of the DHFR gene; the other three lack introns. Transfection of DHFR-deficient Chinese hamster ovary cells with any of these constructed genes results in cells with the DHFR+ phenotype. Treatment of the transfectants with methotrexate, a folate antagonist, leads to the emergence of methotrexate-resistant colonies which have amplified the transfected genes.

MeSH Terms
Animals Base Sequence Cell Line Cricetinae Drug Resistance Gene Amplification Gene Expression Regulation Genes Genetic Engineering Methotrexate/pharmacology Mice Structure-Activity Relationship Tetrahydrofolate Dehydrogenase/genetics
Chemicals
Tetrahydrofolate Dehydrogenase Methotrexate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gasser C S
Simonsen C C
Schilling J W
Schimke R T
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.21.6522
Published
1982-11-00
Pages
6522-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347159
Subset
IM
Grants
NIGMS NIH HHS · GM 14931 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.21.6522 PMC Full Text View journal details
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