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PMID: 6366511 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phenotypic expression in Escherichia coli and nucleotide sequence of two Chinese hamster lung cell cDNAs encoding different dihydrofolate reductases.

Molecular and cellular biology ·Vol. 4 ·No. 1 ·1984-01-00 ·Pages 38-48

Melera PW, Davide JP, Hession CA, Scotto KW

Abstract

Nucleotide sequence analysis of two cDNA clones, one shown to direct the synthesis in Escherichia coli of the pI 6.7 form of the 20,000-molecular-weight class of Chinese hamster lung cell dihydrofolate reductase, and the other shown to direct the synthesis of the pI 6.5 form of the 21,000-molecular-weight class of the enzyme, has revealed the following: (i) the differences in physical and enzymatic properties displayed by these two proteins are due to two variations in their respective amino acid sequences with the conversion of Leu to Phe at position 22 probably responsible for the differential sensitivity of these two enzymes to methotrexate and methasquin; (ii) the multiple mRNAs responsible for the synthesis of each of these proteins differ in size due, at least in part, to a length heterogeneity at their 3' ends; (iii) these two proteins are encoded by different genes; and (iv) the sequence AAATATA appears to be a major polyadenylation signal in one Chinese hamster lung cell dihydrofolate reductase gene and a minor signal in another.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Cell Line Cricetinae Cricetulus/genetics DNA/genetics Escherichia coli/genetics Lung/enzymology Phenotype Tetrahydrofolate Dehydrogenase/genetics
Chemicals
DNA Tetrahydrofolate Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Melera P W
Davide J P
Hession C A
Scotto K W
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.1.38-48.1984
Published
1984-01-00
Pages
38-48
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368655
Subset
IM
Databases
GENBANK
K01163, K01164, K01165
External Links
PubMed source DOI: 10.1128/mcb.4.1.38-48.1984 PMC Full Text View journal details
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