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PMID: 6154933 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Initiation of protein synthesis in bacteria at a translational start codon of mamalian cDNA: effects of the preceding nucleotide sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 3 ·1980-03-00 ·Pages 1442-6

Chang AC, Erlich HA, Gunsalus RP, Nunberg JH, Kaufman RJ, Schimke RT, Cohen SN

Abstract

Plasmids containing a mouse cDNA sequence encoding the enzyme dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) have been used to study the efficiency of initiation of protein synthesis at an ATG (AUG) translational start codon indigenous to the eukaryotic CDNA. differences in DHFR production assayed phenotypically, enzymatically, and immunologically were correlated with the primary structure of the DNA segment that precedes the translational start codon. Our results indicate that initiation of a structurally discrete and biologically functional eukaryotic protein can occur in bacteria on a fused mRNA molecule, and that the efficiency of expression is strongly affected by: (i) the extent of homology of the translational control region with the 3'-OH end of 16S ribosomal RNA, and (ii) the distance between the protein start codon and the ribosome-binding sequence on the mRNA.

MeSH Terms
Animals Base Sequence Codon DNA, Recombinant Epitopes Escherichia coli/genetics Genes Mice Operon Peptide Chain Initiation, Translational RNA, Messenger/genetics Tetrahydrofolate Dehydrogenase/genetics,immunology beta-Lactamases/genetics
Chemicals
Codon DNA, Recombinant Epitopes RNA, Messenger Tetrahydrofolate Dehydrogenase beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chang A C
Erlich H A
Gunsalus R P
Nunberg J H
Kaufman R J
Schimke R T
Cohen S N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.3.1442
Published
1980-03-00
Pages
1442-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348511
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.3.1442 PMC Full Text View journal details
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