Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid.

Nature ·Vol. 275 ·No. 5680 ·1978-10-12 ·Pages 505-10

Mercereau-Puijalon O, Royal A, Cami B, Garapin A, Krust A, Gannon F, Kourilsky P

Abstract

A cloned DNA transcript of ovalbumin mRNA was cut a few nucleotides away from the initiator codon, and fused in phase to the beginning of the Escherichia coli beta-galactosidase gene. The hybrid gene has been cloned in E. coli where it produces large amounts of an ovalbumin-like protein.

MeSH Terms

Codon DNA, Recombinant/metabolism Epitopes Escherichia coli/genetics Lac Operon Ovalbumin/genetics,immunology,metabolism Plasmids Protein Biosynthesis

Chemicals

Codon DNA, Recombinant Epitopes Ovalbumin

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Mercereau-Puijalon O

Royal A

Cami B

Garapin A

Krust A

Gannon F

Kourilsky P

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/275505a0

Published

1978-10-12

Pages

505-10

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/275505a0 View journal details

Analysis Services

Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid.

Contact

WeChat Official Account