Home LiteratureArticle Details
PMID: 6166922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple polyadenylation sites in a mouse alpha-amylase gene.

Nucleic acids research ·Vol. 9 ·No. 10 ·1981-05-25 ·Pages 2313-23

Tosi M, Young RA, Hagenbüchle O, Schibler U

Abstract

Two alpha-amylase mRNAs which differ in the length of their 3' non-translated region accumulate in the cytoplasm in both mouse liver and salivary gland tissues. The two species in each tissue are transcribed from the same gene (Amy-1A). The minor species is approximately 20-nucleotides preceding the poly(A) tract. Sequence analysis of genomic DNA shows that these extra 237 nucleotides are specified by sequences contiguous to those shared by the two mRNAs. These data demonstrate that transcription can proceed through the major polyadenylation site and that alternative polyadenylation sites are used in the Amy-1A gene. Sequences which trail the two polyadenylation sites exhibit extensive homology and might therefore be involved in polyadenylation or transcription termination.

MeSH Terms
Amylases/genetics Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Liver/metabolism Mice Mice, Inbred Strains Nucleic Acid Hybridization Poly A/genetics Protein Biosynthesis RNA, Messenger/genetics Salivary Glands/metabolism alpha-Amylases/genetics
Chemicals
RNA, Messenger Poly A DNA Restriction Enzymes Amylases alpha-Amylases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tosi M
Young R A
Hagenbüchle O
Schibler U
References (21)
21 references, click to expand
  1. Eukaryotic messenger RNA.
    Annu Rev Biochem. 1974;43(0):621-42 PMID: 4604447
  2. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  3. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  4. Processing of RNA.
    Annu Rev Biochem. 1976;45:605-29 PMID: 786158
  5. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  6. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  7. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  8. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  9. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.
    Nature. 1978 Oct 19;275(5681):617-24 PMID: 360074
  10. Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing.
    Cell. 1978 Dec;15(4):1477-93 PMID: 729004
  11. The major late adenovirus type-2 transcription unit: termination is downstream from the last poly(A) site.
    J Mol Biol. 1979 Apr 25;129(4):643-56 PMID: 480353
  12. Comparison of mRNA precursors in plasmacytomas producing closely related kappa chains.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3678-82 PMID: 115006
  13. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  14. Structure and genomic organization of the mouse dihydrofolate reductase gene.
    Cell. 1980 Feb;19(2):355-64 PMID: 6244105
  15. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA.
    Cell. 1980 Mar;19(3):671-81 PMID: 6985473
  16. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  17. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  18. Tissue-specific expression of mouse alpha-amylase genes.
    J Mol Biol. 1980 Sep 5;142(1):93-116 PMID: 6159478
  19. Structure of two adenovirus type 12 transforming polypeptides and their evolutionary implications.
    Nature. 1980 Nov 13;288(5787):174-6 PMID: 7432516
  20. Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells.
    Cell. 1980 Nov;22(2 Pt 2):361-70 PMID: 7448865
  21. Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
    J Mol Biol. 1980 Dec 15;144(3):377-86 PMID: 7253021
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.10.2313
Published
1981-05-25
Pages
2313-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326847
Subset
IM
Databases
GENBANK
J00355
External Links
PubMed source DOI: 10.1093/nar/9.10.2313 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2