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PMID: 6308574 Published · ppublish English Journal Article

The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7.

Nucleic acids research ·Vol. 11 ·No. 15 ·1983-08-11 ·Pages 5147-58

Fling ME, Richards C

Abstract

The complete nucleotide sequence of the type I dihydrofolate reductase gene from Tn7 was determined. The structural gene coded for a polypeptide of 157 amino acid residues. The polypeptide deduced from the DNA sequence had a molecular weight of 17,577 which was in good agreement with that estimated by mobility in SDS-polyacrylamide gels. Sequences were identified proximal to the coding region which were similar to those found in the consensus E. coli promoter region and for the initiation of protein synthesis. Features consistent with the termination of RNA transcription were present distal to the structural gene. No homology was apparent when the DNA sequence of the type I gene was compared to the sequence of the type II plasmid DHFR genes, but sequence homology was evident when the type I and E. coli chromosomal enzymes were compared. Homology was greatest in the regions coding for amino acids which in the E. coli chromosomal enzyme are associated with substrate, cofactor and inhibitor binding.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/drug effects,enzymology Genes/drug effects Genes, Bacterial/drug effects Plasmids Templates, Genetic Tetrahydrofolate Dehydrogenase/genetics Trimethoprim/pharmacology
Chemicals
DNA, Bacterial Trimethoprim Tetrahydrofolate Dehydrogenase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fling M E
Richards C
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.15.5147
Published
1983-08-11
Pages
5147-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326243
Subset
IM
Databases
GENBANK
K00970
External Links
PubMed source DOI: 10.1093/nar/11.15.5147 PMC Full Text View journal details
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