Regulatory mutants of dihydrofolate reductase in Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 147 ·No. 1 ·1976-08-10 ·Pages 91-7

Sheldon R, Brenner S

Abstract

Trimethoprim inhibits dihydrofolate reductase. Mutations conferring trimethorpim-resistance on E coli K12 result in either an altered reductase with decreased affinity for the drug, or in 2-30 fold higher levels of the enzyme. Studies of the latter class of mutants indicate that dihydrofolate reductase is regulatdd by a diffusible molecule, and is probably under negative control. The regulatrory mutants, some of which are temperature-sensitive, act cis.

MeSH Terms

Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Folic Acid Antagonists Genotype Methotrexate/pharmacology Mutation Tetrahydrofolate Dehydrogenase/metabolism Trimethoprim/pharmacology

Chemicals

Folic Acid Antagonists Trimethoprim Tetrahydrofolate Dehydrogenase Methotrexate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sheldon R

Brenner S

References (14)

14 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00337941

Published

1976-08-10

Pages

91-7

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00337941

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Regulatory mutants of dihydrofolate reductase in Escherichia coli K12.

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