Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli.

Amino Acids/analysis Chromatography, Affinity Chromatography, Gel Cyanogen Bromide Dansyl Compounds Drug Resistance, Microbial Electrophoresis Electrophoresis, Disc Escherichia coli/enzymology,growth & development Folic Acid Folic Acid Antagonists Hydrogen-Ion Concentration Methotrexate NAD NADP Osmolar Concentration Potassium Chloride Sodium Chloride Tetrahydrofolate Dehydrogenase/analysis,isolation & purification Ultracentrifugation

Amino Acids Dansyl Compounds Folic Acid Antagonists NAD Sodium Chloride NADP Potassium Chloride Folic Acid Tetrahydrofolate Dehydrogenase Cyanogen Bromide Methotrexate