Methotrexate binding to dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.

Binding Sites Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Folic Acid Folic Acid Antagonists Kinetics Methotrexate/metabolism,pharmacology NADP Osmolar Concentration Oxidation-Reduction Protein Binding Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Tetrahydrofolate Dehydrogenase/metabolism Time Factors

Folic Acid Antagonists NADP Folic Acid Tetrahydrofolate Dehydrogenase Methotrexate