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PMID: 3023925 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 2262-6

Lewis JA, Matkovich DA

Abstract

We have constructed a chimeric thymidine kinase (TK) minigene, pHe delta 6Ha, which combines the complete coding and 3' noncoding regions of a Chinese hamster TK cDNA with the promoter region and 5' untranslated region of the TK gene of herpes simplex virus type 1. We have transformed rat 4 cells to Tk+ with this gene and analyzed the pattern of TK gene expression in these transformants under various conditions of in vitro cell culture. We find that TK gene expression in these Tk+ transformants is growth phase dependent, responsive to adenovirus 5 infection, and indistinguishable in character under a variety of cell culture conditions from the pattern of TK gene expression in rat 4 cells transformed to Tk+ with the genomic Chinese hamster TK gene clone lambda HaTK.5. We are led to the conclusion that the genetic elements which mediate growth phase-dependent TK gene expression are contained entirely within the sequences of the mature cytoplasmic hamster TK mRNA.

MeSH Terms
Adenoviruses, Human/genetics Animals Cell Cycle Cricetinae/genetics Cricetulus/genetics Gene Expression Regulation RNA, Messenger/genetics Simplexvirus/genetics Thymidine Kinase/genetics
Chemicals
RNA, Messenger Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lewis J A
Matkovich D A
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49 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.2262-2266.1986
Published
1986-06-00
Pages
2262-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367769
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.6.2262-2266.1986 PMC Full Text View journal details
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