Home LiteratureArticle Details
PMID: 6199739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Post-transcriptional regulation of the chicken thymidine kinase gene.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1427-46

Groudine M, Casimir C

Abstract

In attempting to understand the molecular basis of the control of chicken thymidine kinase (cTK) gene expression, we have examined the steady state cTK RNA content, and the patterns of DNA methylation, chromatin structure and endogenous nuclear runoff transcription of this gene in dividing and non-dividing cells. Our results reveal that the steady state level of cTK poly A+ RNA is correlated with the divisional activity of normal avian cells and tissues. However, no differences in the pattern of Hpa II site methylation or chromatin structure are found among cells containing high or undetectable levels of steady state cTK RNA. In addition, no differences in cTK transcription as assayed by nuclear runoff experiments are detectable in isolated nuclei derived from dividing or non-dividing cells containing high or low levels of steady state cTK RNA. These results suggest that the principal control of chicken thymidine kinase gene expression is post-transcriptional in nature.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Chick Embryo DNA Restriction Enzymes Fibroblasts/enzymology Genes Nucleic Acid Hybridization Poly A/genetics RNA/genetics,isolation & purification RNA Processing, Post-Transcriptional RNA, Messenger/genetics Thymidine Kinase/genetics Transcription, Genetic
Chemicals
RNA, Messenger Poly A RNA Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Groudine M
Casimir C
References (48)
48 references, click to expand
  1. Variations in phosphokinase activities during the cell cycle in synchronous populations of HeLa cells.
    Nature. 1965 Jul 10;207(993):176-7 PMID: 5886110
  2. Thymidine kinase activity in synchronized HeLa cell cultures.
    Biochem Biophys Res Commun. 1965 Aug 16;20(4):535-8 PMID: 5860172
  3. Initiation of DNA synthesis in a system of synchronized L-cells: rhythmicity of thymidine kinase activity.
    Eur J Biochem. 1968 May;4(4):487-9 PMID: 5660481
  4. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  5. Lineage-dependent transcription of globin genes.
    Cell. 1974 Nov;3(3):243-7 PMID: 4434472
  6. Deoxyribonucleic acid of Marek's disease virus in a lymphoblastoid cell line from Marek's disease tumours.
    J Gen Virol. 1974 Nov;25(2):317-21 PMID: 4474358
  7. Two cell lines from lymphomas of Marek's disease.
    Biken J. 1974 Sep;17(3):105-16 PMID: 4616680
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Formation of thymidine kinase and deoxycytidylate deaminase in synchronized cultures of chinese hamster cells temperature-sensitive for DNA synthesis.
    J Cell Physiol. 1976 May;88(1):57-64 PMID: 1262406
  10. The effect of cell density, conditioned medium and cytosine arabinoside on myogenesis in primary and secondary cultures.
    Exp Cell Res. 1977 Jan;104(1):63-78 PMID: 401740
  11. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  12. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.
    Nucleic Acids Res. 1978 Dec;5(12):4631-4 PMID: 745990
  13. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  14. DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin.
    Nucleic Acids Res. 1979 Dec 20;7(8):2105-13 PMID: 523315
  15. Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Nature. 1980 May 22;285(5762):207-10 PMID: 6246445
  16. Analysis of the myogenic lineage in chick embryos. II. Evidence for a deterministic lineage in the final stages.
    Exp Cell Res. 1980 May;127(1):237-47 PMID: 7379865
  17. Differential nuclease sensitivity of the ovalbumin and beta-globin chromatin regions in erythrocytes and oviduct cells of laying hen.
    Nucleic Acids Res. 1980 Jun 25;8(12):2737-50 PMID: 6253891
  18. DNA methylation and gene function.
    Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
  19. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  20. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
    Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
  21. Cell cycle-dependent regulation of thymidine kinase activity introduced into mouse LMTK- cells by DNA and chromatin-mediated gene transfer.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1119-23 PMID: 6940130
  22. 5-Methylcytosine in eukaryotic DNA.
    Science. 1981 Jun 19;212(4501):1350-7 PMID: 6262918
  23. Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
    Cell. 1981 May;24(2):333-44 PMID: 7237551
  24. Activation of globin genes during chicken development.
    Cell. 1981 May;24(2):393-401 PMID: 7237553
  25. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
    Nature. 1981 Jul 23;292(5821):311-7 PMID: 6166864
  26. Beta + thalassemia: aberrant splicing results from a single point mutation in an intron.
    Cell. 1981 Dec;27(2 Pt 1):289-98 PMID: 6895866
  27. 5-Azacytidine-induced reactivation of a herpes simplex thymidine kinase gene.
    Science. 1982 Apr 2;216(4541):70-3 PMID: 6175023
  28. Regulation of thymidine kinase enzyme level in serum-stimulated mouse 3T6 fibroblasts.
    Exp Cell Res. 1982 Mar;138(1):79-85 PMID: 7067741
  29. Abnormally spliced messenger RNA in erythroid cells from patients with beta+ thalassemia and monkey cells expressing a cloned beta+-thalassemic gene.
    Cell. 1982 Mar;28(3):585-93 PMID: 6280877
  30. Virus persistence and recurring demyelination produced by a temperature-sensitive mutant of MHV-4.
    Nature. 1982 Jul 15;298(5871):279-80 PMID: 6283382
  31. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.
    J Biol Chem. 1982 Aug 10;257(15):8569-72 PMID: 7096324
  32. Control of dihydrofolate reductase messenger ribonucleic acid production.
    Mol Cell Biol. 1981 Nov;1(11):961-71 PMID: 6180296
  33. Propagation of globin DNAase I-hypersensitive sites in absence of factors required for induction: a possible mechanism for determination.
    Cell. 1982 Aug;30(1):131-9 PMID: 6290075
  34. Expression of transferred thymidine kinase genes is controlled by methylation.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6299-303 PMID: 6183659
  35. Modulation of transfected gene expression mediated by changes in chromatin structure.
    Cell. 1982 Dec;31(3 Pt 2):521-9 PMID: 6186385
  36. Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression.
    Mol Cell Biol. 1982 Nov;2(11):1304-19 PMID: 6131378
  37. Simultaneous analysis of conformation and transcription of A and B groups of vitellogenin genes in male and female Xenopus during primary and secondary activation by estrogen.
    Nucleic Acids Res. 1983 Feb 25;11(4):1151-66 PMID: 6687495
  38. Expression of c-myc RNA in bursal lymphoma cell lines: identification of c-myc-encoded proteins by hybrid-selected translation.
    Cell. 1983 Jun;33(2):335-44 PMID: 6190569
  39. Is apparent autoregulatory control of tubulin synthesis nontranscriptionally regulated?
    J Cell Biol. 1983 Sep;97(3):919-24 PMID: 6885926
  40. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  41. Delayed processing of dihydrofolate reductase heterogeneous nuclear RNA in amino acid-starved mouse fibroblasts.
    Mol Cell Biol. 1983 Oct;3(10):1792-802 PMID: 6646125
  42. Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
    Cell. 1983 May;33(1):65-76 PMID: 6088056
  43. Transcriptional regulation of hemoglobin switching in chicken embryos.
    Mol Cell Biol. 1981 Mar;1(3):281-8 PMID: 6086008
  44. STUDIES ON THYMIDINE KINASE IN CULTURED MOUSE FIBROBLASTS.
    Biochim Biophys Acta. 1965 Jan 11;95:14-22 PMID: 14289021
  45. DECLINE OF THYMIDINE KINASE ACTIVITY IN STATIONARY PHASE MOUSE FIBROBLAST CELLS.
    J Biol Chem. 1965 Jun;240:2565-73 PMID: 14304868
  46. ACTIVITIES OF THYMIDINE KINASE AND THYMINE DEOXYRIBONUCLEOTIDE PHOSPHATASE DURING GROWTH OF CELLS IN TISSUE CULTURE.
    J Biol Chem. 1965 Jun;240:2607-11 PMID: 14304875
  47. Phenotypic switching in cells transformed with the herpes simplex virus thymidine kinase gene.
    Mol Cell Biol. 1982 Jun;2(6):708-14 PMID: 14582166
  48. The periodic synthesis of thymidine kinase in mouse fibroblasts.
    Biochim Biophys Acta. 1966 Feb 21;114(2):398-403 PMID: 4223355
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.3.1427
Published
1984-02-10
Pages
1427-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318587
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.3.1427 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2