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PMID: 6180296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Control of dihydrofolate reductase messenger ribonucleic acid production.

Molecular and cellular biology ·Vol. 1 ·No. 11 ·1981-11-00 ·Pages 961-71

Leys EJ, Kellems RE

Abstract

We used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene expression. As a result of growth stimulation, the relative rate of dihydrofolate reductase protein synthesis increased threefold, reaching a maximum between 25 and 30 h after stimulation. The relative rate of dihydrofolate reductase messenger ribonucleic acid production (i.e., the appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm) increased threefold after growth stimulation and was accompanied by a corresponding increase in the relative steady-state level of dihydrofolate reductase ribonucleic acid in the nucleus. However, the increase in the nuclear level of dihydrofolate reductase ribonucleic acid was not accompanied by a significant increase in the relative rate of transcription of the dihydrofolate reductase genes. These data indicated that the relative rate of appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm depends on the relative stability of the dihydrofolate reductase ribonucleic acid sequences in the nucleus and is not dependent on the relative rate of transcription of the dihydrofolate reductase genes.

MeSH Terms
Animals Cell Division Cell Nucleus/metabolism Cells, Cultured Cytoplasm/metabolism DNA, Recombinant Drug Resistance Methotrexate/pharmacology Mice Nucleic Acid Hybridization Poly A/analysis RNA/analysis RNA, Messenger/metabolism Sarcoma 180 Tetrahydrofolate Dehydrogenase/genetics
Chemicals
DNA, Recombinant RNA, Messenger Poly A RNA Tetrahydrofolate Dehydrogenase Methotrexate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leys E J
Kellems R E
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.11.961-971.1981
Published
1981-11-00
Pages
961-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369718
Subset
IM
Grants
NCI NIH HHS · CA24618 · United States
NCRR NIH HHS · RR-05425 · United States
External Links
PubMed source DOI: 10.1128/mcb.1.11.961-971.1981 PMC Full Text View journal details
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