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PMID: 2427924 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Adenovirus type 2 activates cell cycle-dependent genes that are a subset of those activated by serum.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 2936-42

Liu HT, Baserga R, Mercer WE

Abstract

We have studied a panel of 10 genes and cDNA sequences that are expressed in a cell cycle-dependent manner in different types of cells from different species and that are inducible by different mitogens. These include five sequences (c-myc, 4F1, 2F1, 2A9, and KC-1) that are preferentially expressed in the early part of the G1 phase, three genes (ornithine decarboxylase, p53, and c-rasHa) preferentially expressed in middle or late G1, and two genes (thymidine kinase and histone H3) preferentially expressed in the S phase of the cell cycle. We have studied the expression of these genes in nonpermissive (tsAF8) and semipermissive (Swiss 3T3) cells infected with adenovirus type 2. Under the conditions of these experiments, adenovirus type 2 infection stimulates cellular DNA synthesis in both tsAF8 and 3T3 cells. However, four of the five early G1 genes (c-myc, 4F1, KC-1, and 2A9) and one of the late G1 genes (c-ras) are not induced by adenovirus infection, although they are strongly induced by serum. The other sequences (2F1, ornithine decarboxylase, p53, thymidine kinase, and histone H3) are activated by both adenovirus and serum. We conclude that the cell cycle-dependent genes activated by adenovirus 2 are a subset of the cell cycle-dependent genes activated by serum. The data suggest that the mechanisms by which serum and adenovirus induce cellular DNA synthesis are not identical.

MeSH Terms
Adenoviruses, Human/genetics Animals Blood Cell Cycle Cell Line Cell Transformation, Viral Cells, Cultured Cricetinae Culture Media DNA/analysis DNA Replication Genes HeLa Cells/cytology Histones/genetics Kidney Mutation Plasmids RNA/genetics Temperature Thymidine Kinase/genetics
Chemicals
Culture Media Histones RNA DNA Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liu H T
Baserga R
Mercer W E
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65 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.2936-2942.1985
Published
1985-11-00
Pages
2936-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369104
Subset
IM
Grants
NIA NIH HHS · AG-00378 · United States
NCI NIH HHS · CA-25898 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.11.2936-2942.1985 PMC Full Text View journal details
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