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PMID: 6513920 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of a cell cycle mutant to delineate the critical period for the control of histone mRNA levels in the mammalian cell cycle.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2364-9

Artishevsky A, Delegeane AM, Lee AS

Abstract

Temporal analysis of DNA replication and histone mRNA accumulation in a hamster fibroblast cell cycle mutant (K12) showed that histone mRNA accumulates periodically during the cell cycle and reaches its highest level in the S phase. The direct correlation between the initiation of DNA synthesis and the accumulation of histone mRNA to high levels in S phase demonstrated the strict interdependence of these two events. Moreover, a critical period necessary for histone mRNA accumulation occurred late in G1 phase. If cells were incubated at the nonpermissive temperature during this critical period, the amount of histone mRNA remained at the basal level. Transcription rate measurements indicated that the triggering of histone mRNA synthesis occurred in late G1 and this mRNA was synthesized at its maximal rate 3 to 5 h before its peak of accumulation. However, if cells were prohibited from synthesizing DNA as a consequence of the temperature-sensitive block in G1, the synthesis of histone mRNA was not initiated.

MeSH Terms
Animals Cell Cycle Cells, Cultured Cricetinae Gene Expression Regulation Histones/genetics Mutation RNA, Messenger/genetics,metabolism Temperature Time Factors Transcription, Genetic
Chemicals
Histones RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Artishevsky A
Delegeane A M
Lee A S
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2364-2369.1984
Published
1984-11-00
Pages
2364-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369066
Subset
IM
Grants
NIGMS NIH HHS · GM 31138 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.11.2364-2369.1984 PMC Full Text View journal details
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