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PMID: 6315396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1633-9

Oren M, Bienz B, Givol D, Rechavi G, Zakut R

Abstract

Three cDNA clones, corresponding to two non-overlapping regions of the mRNA coding for the mouse p53 cellular tumor antigen, were isolated and characterized. In hybridization-selection assays, these clones were capable of selectively binding p53 mRNA, as demonstrated by in vitro translation and immunoprecipitation with anti-p53 monoclonal antibodies. The p53 mRNA appeared to be the only messenger species specifically selected by these clones. The size of the p53 mRNA was found to be approximately 2 kb, and its levels to vary substantially among different types of transformed cells. Evidence was found for the existence of two distinct p53-specific genes in mouse genomic DNA. Two partially overlapping recombinant phage clones were obtained, both derived from the same p53-specific genomic DNA region. The orientation of the various cDNA clones relative to that of the p53 mRNA was established by S1 analysis and the relationship between the cDNA clones and the genomic ones was determined by comparative restriction enzyme mapping and nucleic acid hybridization.

MeSH Terms
Animals Cloning, Molecular DNA/analysis DNA Restriction Enzymes DNA, Recombinant/analysis Embryo, Mammalian Mice Mice, Inbred BALB C Molecular Weight Neoplasm Proteins/genetics Nucleic Acid Hybridization Phosphoproteins/genetics Tumor Suppressor Protein p53
Chemicals
DNA, Recombinant Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53 DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oren M
Bienz B
Givol D
Rechavi G
Zakut R
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1633-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555338
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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