Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 1 ·1981-01-00 ·Pages 41-5

Crawford LV, Pim DC, Gurney EG, Goodfellow P, Taylor-Papadimitriou J

Abstract

Human cell lines, whether derived from spontaneous tumors or transformed in vitro with simian virus 40, were found to contain a 53,000-dalton phosphoprotein (pp53) in contrast to normal human cells in which this protein was not detected. Isoelectric focusing showed that pp53 comprised several species in both simian virus 40-transformed and tumor cells. Comparison of the pp53 species from the various cell lines by partial proteolysis showed that they were similar but not identical. Among the 13 tumor cell lines examined, only 1 line, HeLa, did not contain detectable pp53. All the other tumor cell lines contained pp53, and it is suggested that this protein may be associated with their transformed state.

MeSH Terms

Antigens, Neoplasm/immunology Antigens, Viral/immunology Antigens, Viral, Tumor Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral HeLa Cells Humans Neoplasm Proteins/immunology,isolation & purification Neoplasms/analysis Phosphoproteins/immunology,isolation & purification Precipitin Tests Simian virus 40 Tumor Suppressor Protein p53

Chemicals

Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Crawford L V

Pim D C

Gurney E G

Goodfellow P

Taylor-Papadimitriou J

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.1.41

Published

1981-01-00

Pages

41-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC318985

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.1.41 PMC Full Text View journal details

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Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.

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