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PMID: 3839456 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.

The EMBO journal ·Vol. 4 ·No. 2 ·1985-02-00 ·Pages 437-43

Valerio D, Duyvesteyn MG, Dekker BM, Weeda G, Berkvens TM, van der Voorn L, van Ormondt H, van der Eb AJ

Abstract

Cosmid clones containing the gene for human adenosine deaminase (ADA) were isolated. The gene is 32 kb long and split into 12 exons. The exact sizes and boundaries of the exon blocks including the transcription start sites were determined. The sequence upstream from this cap site lacks the TATA and CAAT boxes characteristic for eukaryotic promoters. Nevertheless, we have shown in a functional assay that a stretch of 135 bp immediately preceding the cap site has promoter activity. This 135-bp DNA fragment is extremely rich in G/C residues (82%). It contains three inverted repeats that allow the formation of cruciform structures, a 10-bp and a 16-bp direct repeat and five G/C-rich motifs (GGGCGGG) disposed in a strikingly symmetrical fashion. Some of these structural features were also found in the promoter region of other genes and we discuss their possible function. Knowledge of the exact positions of the intron-exon boundaries allowed us to propose models for abnormal RNA processing that occurs in previously investigated ADA-deficient cell lines.

MeSH Terms
Adenosine Deaminase/genetics Base Sequence Cells, Cultured DNA/genetics DNA, Recombinant Gene Expression Regulation Genes Humans Nucleic Acid Conformation Nucleoside Deaminases/genetics Promoter Regions, Genetic RNA, Messenger/genetics
Chemicals
DNA, Recombinant RNA, Messenger DNA Nucleoside Deaminases Adenosine Deaminase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Valerio D
Duyvesteyn M G
Dekker B M
Weeda G
Berkvens T M
van der Voorn L
van Ormondt H
van der Eb A J
References (39)
39 references, click to expand
  1. Adenosine-deaminase deficiency in two patients with severely impaired cellular immunity.
    Lancet. 1972 Nov 18;2(7786):1067-9 PMID: 4117384
  2. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  3. Assignment of a gene for adenosine deaminase to human chromosome 20.
    Hum Hered. 1974;24(1):1-11 PMID: 4136545
  4. Human adenosine deaminase. Purification and subunit structure.
    J Biol Chem. 1977 Jan 10;252(1):110-5 PMID: 13062
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  7. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. Adenosine deaminase deficiency and severe combined immunodeficiency disease.
    Adv Enzymol Relat Areas Mol Biol. 1980;51:167-210 PMID: 6255772
  10. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  11. Sequences of human adenovirus Ad3 and Ad7 DNAs encoding the promoter and first leader segment of late RNAs.
    Gene. 1981 Mar;13(2):133-43 PMID: 6263757
  12. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  13. Biochemistry of diseases of immunodevelopment.
    Annu Rev Biochem. 1981;50:845-77 PMID: 6267992
  14. Structure of the promoter for chicken alpha 2 type I collagen gene.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5334-8 PMID: 6946474
  15. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  16. The construction of cosmid libraries which can be used to transform eukaryotic cells.
    Nucleic Acids Res. 1982 Nov 11;10(21):6715-32 PMID: 6294612
  17. Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in mgh-mouse and man--Chinese hamster somatic cell hybrids.
    Arch Biochem Biophys. 1971 Aug;145(2):470-83 PMID: 4331030
  18. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  19. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  20. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  21. The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.
    Nucleic Acids Res. 1983 Apr 25;11(8):2447-64 PMID: 6304653
  22. A zein gene of maize is transcribed from two widely separated promoter regions.
    Cell. 1983 Oct;34(3):1015-22 PMID: 6313202
  23. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  24. Molecular cloning of human adenosine deaminase gene sequences.
    J Biol Chem. 1983 Nov 10;258(21):12753-6 PMID: 6688808
  25. Isolation of cDNA clones for human adenosine deaminase.
    Gene. 1983 Nov;25(2-3):231-40 PMID: 6198240
  26. Adenosine deaminase (ADA) deficiency in cells derived from humans with severe combined immunodeficiency is due to an aberration of the ADA protein.
    Nucleic Acids Res. 1984 Jan 25;12(2):1015-24 PMID: 6198631
  27. Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters.
    Nucleic Acids Res. 1984 Jan 25;12(2):915-32 PMID: 6320125
  28. Cloning of cDNA sequences of human adenosine deaminase.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7481-5 PMID: 6200875
  29. A detailed analysis of an HSV-1 early promoter: sequences involved in trans-activation by viral immediate-early gene products are not early-gene specific.
    Nucleic Acids Res. 1984 Apr 11;12(7):3037-56 PMID: 6326049
  30. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
  31. The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence.
    Cell. 1984 May;37(1):253-62 PMID: 6233005
  32. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  33. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.
    Cell. 1984 Aug;38(1):275-85 PMID: 6088070
  34. Human adenosine deaminase. cDNA and complete primary amino acid sequence.
    J Biol Chem. 1984 Oct 10;259(19):12101-6 PMID: 6090454
  35. Analysis of the gene coding for the murine cellular tumour antigen p53.
    EMBO J. 1984 Sep;3(9):2179-83 PMID: 6092064
  36. Structure of adenosine deaminase mRNAs from normal and adenosine deaminase-deficient human cell lines.
    Mol Cell Biol. 1984 Sep;4(9):1712-7 PMID: 6208479
  37. Genetic and physical analysis of the chicken tk gene.
    Mol Cell Biol. 1984 Sep;4(9):1769-76 PMID: 6092937
  38. Effect of the 21-bp repeat upstream element on in vitro transcription from the early and late SV40 promoters.
    EMBO J. 1984 Oct;3(10):2373-82 PMID: 6094181
  39. Cloning of human adenosine deaminase cDNA and expression in mouse cells.
    Gene. 1984 Nov;31(1-3):147-53 PMID: 6526272
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-02-00
Pages
437-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554205
Subset
IM
Databases
GENBANK
M11816, M11817, M11818, M11819, M11820, M11821, M11822, M11823, M11824, M11825, M11826, X02994
External Links
PubMed source PMC Full Text View journal details
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