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PMID: 3141919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 7857-61

Christy BA, Lau LF, Nathans D

Abstract

We have recently identified by cDNA cloning a set of genes that are rapidly activated in mouse 3T3 cells by serum or purified growth factors. Here we report that the cDNA (clone 268) derived from one of these immediate early genes (zif/268) encodes a protein with three tandem "zinc finger" sequences typical of a class of eukaryotic transcription factors. The mRNA of zif/268 is present in many organs and tissues of the mouse and is especially abundant in the brain and thymus tissue. The 5' genomic flanking sequence of zif/268 has sequences related to binding sites for known regulatory proteins, including four sequences that resemble the core of the serum response elements (SREs) upstream of the c-fos and actin genes. The SRE-like sequences could be responsible for the coordinate activation of zif/268 and fos after serum stimulation of 3T3 cells.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line/drug effects DNA/analysis DNA-Binding Proteins/genetics Gene Expression Regulation Growth Substances/pharmacology Male Metalloproteins/genetics Mice Mice, Inbred BALB C Molecular Sequence Data Nuclear Proteins/genetics Plasmids RNA, Messenger/analysis Serum Response Factor
Chemicals
DNA-Binding Proteins Growth Substances Metalloproteins Nuclear Proteins RNA, Messenger Serum Response Factor DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Christy B A
Howard Hughes Medical Institute Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Lau L F
Nathans D
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46 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.7857
Published
1988-11-00
Pages
7857-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282296
Subset
IM
Grants
NCI NIH HHS · 5 P01 CA16519 · United States
Databases
GENBANK
J04089, M22326, M22327
External Links
PubMed source DOI: 10.1073/pnas.85.21.7857 PMC Full Text View journal details
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