Home LiteratureArticle Details
PMID: 3174663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Convulsant-induced increase in transcription factor messenger RNAs in rat brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 20 ·1988-10-00 ·Pages 7795-9

Saffen DW, Cole AJ, Worley PF, Christy BA, Ryder K, Baraban JM

Abstract

Administration of the convulsants pentylenetetrazole (Metrazole) or picrotoxin to rats caused a dramatic increase in mRNAs of four putative transcription factor genes, zif/268, c-jun, jun-B, and c-fos, in neurons of the hippocampus and dentate gyrus, as well as other areas of the cerebral cortex, including pyriform cortex and cingulate cortex. The increase in these mRNAs was rapid and transient: amounts peaked within 1 hr and returned to baseline within 2 hr. These results extend the observation made by Morgan et al. [Morgan, J. I., Cohen, D. R., Hempstead, J. L. & Curran, T. (1987) Science 237, 192-197] that c-fos mRNA and protein are induced in rat brain after seizures. We hypothesize that the increase of these putative transcription factor mRNAs in the brain is part of a programmed genomic response of neurons to intense stimulation, which is analogous to the genomic response of nonneuronal cells to growth factors.

MeSH Terms
Animals Autoradiography Blotting, Northern Brain/drug effects,metabolism Convulsants/pharmacology Gene Expression Regulation/drug effects Male Neurons/analysis Nucleic Acid Hybridization Pentylenetetrazole/pharmacology Picrotoxin/pharmacology RNA, Messenger/biosynthesis Rats Rats, Inbred Strains Transcription Factors/genetics
Chemicals
Convulsants RNA, Messenger Transcription Factors Picrotoxin Pentylenetetrazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Saffen D W
Howard Hughes Medical Institute Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Cole A J
Worley P F
Christy B A
Ryder K
Baraban J M
References (36)
36 references, click to expand
  1. Actions of drugs that deplete serotonin.
    Fed Proc. 1977 Jul;36(8):2149-53 PMID: 141378
  2. A novel early growth response gene rapidly induced by fibroblast, epithelial cell and lymphocyte mitogens.
    Oncogene Res. 1987 Sep-Oct;1(4):343-55 PMID: 3130602
  3. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  4. Molecular cloning of gene sequences regulated by platelet-derived growth factor.
    Cell. 1983 Jul;33(3):939-47 PMID: 6872001
  5. Growth-related changes in specific mRNAs of cultured mouse cells.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4271-5 PMID: 6576337
  6. c-fos protein can induce cellular transformation: a novel mechanism of activation of a cellular oncogene.
    Cell. 1984 Jan;36(1):51-60 PMID: 6607118
  7. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  8. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  9. Blockade of long-term potentiation in rat hippocampal CA1 region by inhibitors of protein synthesis.
    J Neurosci. 1984 Dec;4(12):3080-8 PMID: 6502226
  10. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  11. Blockade of norepinephrine-induced long-lasting potentiation in the hippocampal dentate gyrus by an inhibitor of protein synthesis.
    Brain Res. 1985 Dec 30;361(1-2):276-83 PMID: 4084800
  12. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.
    EMBO J. 1985 Dec 1;4(12):3145-51 PMID: 3841511
  13. The long and the short of long-term memory--a molecular framework.
    Nature. 1986 Jul 31-Aug 6;322(6078):419-22 PMID: 2874497
  14. Role of ion flux in the control of c-fos expression.
    Nature. 1986 Aug 7-13;322(6079):552-5 PMID: 2426600
  15. Stimulation of neuronal acetylcholine receptors induces rapid gene transcription.
    Science. 1986 Oct 3;234(4772):80-3 PMID: 3749894
  16. Second messenger dualism in neuromodulation and memory.
    Nature. 1986 Sep 25-Oct 1;323(6086):294-5 PMID: 3762676
  17. A critical period for macromolecular synthesis in long-term heterosynaptic facilitation in Aplysia.
    Science. 1986 Dec 5;234(4781):1249-54 PMID: 3775383
  18. Tissue localization of Drosophila melanogaster ras transcripts during development.
    Mol Cell Biol. 1986 Jun;6(6):2241-8 PMID: 3097510
  19. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6 PMID: 3469660
  20. Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3316-9 PMID: 3554236
  21. Avian sarcoma virus 17 carries the jun oncogene.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2848-52 PMID: 3033666
  22. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  23. Mapping patterns of c-fos expression in the central nervous system after seizure.
    Science. 1987 Jul 10;237(4811):192-7 PMID: 3037702
  24. Induction of c-fos-like protein in spinal cord neurons following sensory stimulation.
    Nature. 1987 Aug 13-19;328(6131):632-4 PMID: 3112583
  25. Kindling stimulation induces c-fos protein(s) in granule cells of the rat dentate gyrus.
    Nature. 1987 Oct 1-7;329(6138):441-2 PMID: 3116433
  26. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  27. The fos oncogene.
    Adv Cancer Res. 1987;49:29-52 PMID: 2823543
  28. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  29. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
    Cell. 1987 Dec 24;51(6):1079-90 PMID: 3319186
  30. Differential regulation of neuropeptide and proto-oncogene mRNA content in the hippocampus following recurrent seizures.
    Brain Res. 1987 Dec;427(1):21-9 PMID: 3427445
  31. A gene activated by growth factors is related to the oncogene v-jun.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 PMID: 3422745
  32. Zinc fingers: gilt by association.
    Cell. 1988 Jan 15;52(1):1-3 PMID: 3125980
  33. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
  34. G protein mRNA mapped in rat brain by in situ hybridization.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2864-8 PMID: 3128797
  35. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
    EMBO J. 1988 Jan;7(1):29-35 PMID: 3129290
  36. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.20.7795
Published
1988-10-00
Pages
7795-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282280
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.20.7795 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2