Home LiteratureArticle Details
PMID: 3129290 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.

The EMBO journal ·Vol. 7 ·No. 1 ·1988-01-00 ·Pages 29-35

Chavrier P, Zerial M, Lemaire P, Almendral J, Bravo R, Charnay P

Abstract

Zinc fingers are DNA-binding domains present in several eukaryotic regulatory proteins. We have identified a mouse gene, Krox-20, encoding a protein with three zinc fingers and whose expression is activated during G0/G1 transition in cultured cells. Serum stimulation of quiescent cells leads to rapid and transient accumulation of Krox-20 mRNA, with kinetics similar to those of the c-fos proto-oncogene. The induction does not require de-novo protein synthesis. In the mouse, Krox-20 is expressed at low levels in tissues which contain rapidly dividing cells. These properties suggest that Krox-20 encodes a transcription control factor, possibly involved in the modulation of cell proliferation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA/genetics DNA-Binding Proteins/genetics,metabolism Early Growth Response Protein 2 Gene Expression Regulation Genes Interphase Metalloproteins/genetics Mice Mice, Inbred Strains Molecular Sequence Data Nucleic Acid Hybridization Transcription Factors/genetics Zinc
Chemicals
DNA-Binding Proteins Early Growth Response Protein 2 Egr2 protein, mouse Metalloproteins Transcription Factors DNA Zinc
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chavrier P
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, FRG.
Zerial M
Lemaire P
Almendral J
Bravo R
Charnay P
References (64)
64 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Activation and repression of mammalian gene expression by the c-myc protein.
    Genes Dev. 1987 Jun;1(4):347-57 PMID: 3678827
  3. Animal cell cycle.
    Annu Rev Biochem. 1978;47:715-50 PMID: 354504
  4. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  5. Coding potential and regulatory signals of the polyoma virus genome.
    Nature. 1980 Jan 31;283(5746):445-53 PMID: 6243401
  6. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
    Cell. 1980 Jan;19(1):13-25 PMID: 7357599
  7. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
    Cell. 1980 Jan;19(1):27-35 PMID: 7357604
  8. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
    Cell. 1980 Mar;19(3):717-28 PMID: 6153931
  9. The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
    Cell. 1981 Mar;23(3):665-9 PMID: 7226225
  10. Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
    Cell. 1982 Dec;31(2 Pt 1):395-405 PMID: 6297765
  11. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  12. Molecular cloning of gene sequences regulated by platelet-derived growth factor.
    Cell. 1983 Jul;33(3):939-47 PMID: 6872001
  13. Expression of the large T protein of polyoma virus promotes the establishment in culture of "normal" rodent fibroblast cell lines.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4354-8 PMID: 6308618
  14. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  15. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  16. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
  17. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  18. Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins.
    Nature. 1984 Jul 5-11;310(5972):70-1 PMID: 6429549
  19. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
    EMBO J. 1984 Jun;3(6):1429-34 PMID: 6086324
  20. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  21. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  22. Human DNA sequences homologous to a protein coding region conserved between homeotic genes of Drosophila.
    Cell. 1984 Oct;38(3):667-73 PMID: 6091895
  23. Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor.
    Science. 1984 Nov 30;226(4678):1080-2 PMID: 6093261
  24. Regulation of heat shock protein 70 gene expression by c-myc.
    Nature. 1984 Nov 15-21;312(5991):280-2 PMID: 6438521
  25. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  26. Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
    Cell. 1984 Dec;39(3 Pt 2):479-89 PMID: 6210149
  27. Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):711-6 PMID: 6514007
  28. Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):716-20 PMID: 6334806
  29. Molecular genetics of Krüppel, a gene required for segmentation of the Drosophila embryo.
    Nature. 1985 Jan 3-9;313(5997):27-32 PMID: 3917552
  30. Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
    Nature. 1985 Feb 21-27;313(6004):703-6 PMID: 2579337
  31. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  32. Structural analysis of murine genes containing homoeo box sequences and their expression in embryonal carcinoma cells.
    Nature. 1985 Apr 25-May 1;314(6013):713-8 PMID: 2986010
  33. The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
    FEBS Lett. 1985 Jul 8;186(2):271-4 PMID: 4007166
  34. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  35. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  36. A mouse homoeo box gene is expressed during embryogenesis and in adult kidney.
    Nature. 1985 Oct 24-30;317(6039):745-8 PMID: 4058581
  37. Sequences from polyomavirus and simian virus 40 large T genes capable of immortalizing primary rat embryo fibroblasts.
    J Virol. 1985 Dec;56(3):958-68 PMID: 2999449
  38. Expression during embryogenesis of a mouse gene with sequence homology to the Drosophila engrailed gene.
    Cell. 1985 Nov;43(1):29-37 PMID: 2416459
  39. Homeo box gene complex on mouse chromosome 11: molecular cloning, expression in embryogenesis, and homology to a human homeo box locus.
    Cell. 1985 Nov;43(1):9-18 PMID: 3000607
  40. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  41. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.
    EMBO J. 1985 Dec 1;4(12):3145-51 PMID: 3841511
  42. Transcriptional activation encoded by the v-fos gene.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3213-7 PMID: 3010284
  43. At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.
    J Mol Biol. 1987 Aug 20;196(4):947-50 PMID: 3681984
  44. An underlying repeat in some transcriptional control sequences corresponding to half a double helical turn of DNA.
    Cell. 1986 Jul 4;46(1):123-32 PMID: 3013416
  45. V-fos stimulates expression of the alpha 1(III) collagen gene in NIH 3T3 cells.
    Biochem Biophys Res Commun. 1986 May 14;136(3):1042-8 PMID: 2424437
  46. A mouse homeo box gene is expressed in spermatocytes and embryos.
    Science. 1986 Aug 8;233(4764):663-7 PMID: 3726554
  47. Isolation of a mouse cDNA coding for a developmentally regulated, testis-specific transcript containing homeo box homology.
    EMBO J. 1986 Jun;5(6):1229-35 PMID: 2426103
  48. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  49. A new homeo-box is present in overlapping cosmid clones which define the mouse Hox-1 locus.
    EMBO J. 1986 Aug;5(8):1973-80 PMID: 3019676
  50. A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.
    Cell. 1986 Dec 26;47(6):1025-32 PMID: 3096579
  51. Fingers and helices.
    Nature. 1986 Nov 20-26;324(6094):215 PMID: 3785392
  52. A multigene family encoding several "finger" structures is present and differentially active in mammalian genomes.
    Cell. 1987 Mar 13;48(5):771-8 PMID: 3815523
  53. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6 PMID: 3469660
  54. Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
    Nature. 1987 Apr 2-8;326(6112):515-7 PMID: 2882423
  55. Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein.
    Nature. 1987 Apr 2-8;326(6112):517-20 PMID: 2882424
  56. Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins.
    Proc Natl Acad Sci U S A. 1987 Mar;84(6):1575-9 PMID: 2882507
  57. Bombesin induces c-fos and c-myc expression in quiescent Swiss 3T3 cells. Comparative study with other mitogens.
    Exp Cell Res. 1987 May;170(1):103-15 PMID: 3494622
  58. Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos.
    Cell. 1987 Jun 19;49(6):835-44 PMID: 3555845
  59. Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein.
    Nature. 1987 Jul 23-29;328(6128):353-5 PMID: 3299106
  60. Metal-dependent folding of a single zinc finger from transcription factor IIIA.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4841-5 PMID: 3474629
  61. Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function.
    Nature. 1987 Jul 30-Aug 5;328(6129):443-5 PMID: 3112579
  62. Regulation of c-fos gene expression in hamster fibroblasts: initiation and elongation of transcription and mRNA degradation.
    Nucleic Acids Res. 1987 Jul 24;15(14):5657-67 PMID: 3615200
  63. Specific stimulation of simian virus 40 late transcription in vitro by a cellular factor binding the simian virus 40 21-base-pair repeat promoter element.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6025-9 PMID: 2819862
  64. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-01-00
Pages
29-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454212
Subset
IM
Databases
GENBANK
X06746
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2