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PMID: 3119326 Published · ppublish English Journal Article

Identification and purification of a polypeptide that binds to the c-fos serum response element.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2711-7

Treisman R

Abstract

A short DNA sequence element, the serum response element (SRE), which binds a nuclear protein, serum response factor (SRF), mediates transient transcriptional activation of c-fos and cytoskeletal actin genes in response to serum factors. Variant SRE sequences with different affinities for HeLa cell SRF were synthesised. Binding of SRF to these sites in vitro correlates with the transcriptional properties of these elements in vivo, suggesting that SRF is a positively acting transcription factor. A 67-kd polypeptide was identified as the DNA-binding component of SRF by photoactivated DNA-protein cross-linking in vitro. The high affinity SRF-binding site was used to purify this polypeptide to virtual homogeneity in a single DNA affinity chromatography step.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Cells, Cultured DNA/metabolism DNA-Binding Proteins/isolation & purification,metabolism Genetic Variation HeLa Cells/metabolism Humans Plasmids Protein Binding Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogenes
Chemicals
DNA-Binding Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Treisman R
MRC Laboratory of Molecular Biology, Cambridge, UK.
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2711-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553694
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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