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PMID: 2582236 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1058-66

Iba H, Cross FR, Garber EA, Hanafusa H

Abstract

We have previously found that Rous sarcoma virus variants in which the viral src (v-src) gene is replaced by the cellular src (c-src) gene have no transforming activity. In this study, we analyzed the basis for the inability of the p60c-src overproduced by these variants to transform cells. Phosphorylations of tyrosine residues in total cell protein or in cellular 34K protein are known to be markedly enhanced upon infection with wild-type Rous sarcoma virus. We found that these tyrosine phosphorylations were only slightly increased in the c-src-containing virus-infected cells, whereas both levels were significantly increased by infection with wild-type Rous sarcoma virus, or transforming mutant viruses which are derived from c-src-containing viruses by spontaneous mutation. Phosphorylation at tyrosine 416 of p60 itself was also extremely low in overproduced p60c-src and high in p60s of transforming mutant viruses. In immunoprecipitates with monoclonal antibody, the overproduced p60c-src had much lower casein tyrosine kinase activity than did p60v-src. We previously showed that p60 myristylation and plasma membrane localization may be required for cell transformation. p60c-src was similar to transforming p60s in these properties. These results strongly suggest that the low level of tyrosine phosphorylation by overproduced p60c-src accounts for its inability to transform cells.

MeSH Terms
Animals Antigen-Antibody Complex Avian Sarcoma Viruses/genetics Cell Compartmentation Cell Membrane/metabolism Cell Transformation, Viral Chick Embryo Macromolecular Substances Molecular Weight Mutation Myristic Acid Myristic Acids/metabolism Peptide Fragments/analysis Phosphoproteins/metabolism Phosphorylation Protein Kinases/metabolism Protein Processing, Post-Translational Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src) Tyrosine/metabolism
Chemicals
Antigen-Antibody Complex Macromolecular Substances Myristic Acids Peptide Fragments Phosphoproteins Myristic Acid Tyrosine Protein Kinases Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iba H
Cross F R
Garber E A
Hanafusa H
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55 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1058-1066.1985
Published
1985-05-00
Pages
1058-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366822
Subset
IM
Grants
NIAID NIH HHS · AI07233 · United States
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · CA18213 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.5.1058-1066.1985 PMC Full Text View journal details
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