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PMID: 6292477 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src.

Journal of virology ·Vol. 44 ·No. 1 ·1982-10-00 ·Pages 1-11

Takeya T, Feldman RA, Hanafusa H

Abstract

Recovered avian sarcoma virus is a class of virus obtained from chicken tumors induced by mutants of Rous sarcoma virus which have a deletion in the src gene. We have determined the entire nucleotide sequence of a 3.1-kilobase EcoRI DNA fragment of molecularly cloned recovered avian sarcoma virus DNA. This DNA fragment contains part of the env gene and the entire src gene. Amino acid sequences of both gene products were deduced from the DNA sequences; the predicted amino acid sequences were verified by protein studies. An env protein (gp37) was found to be composed of 205 amino acids with three glycosylation sites. gp37 had a long stretch of hydrophobic residues near the carboxyl terminus. The src gene product, pp60src, was composed of 526 amino acids and contained the possible sites for tyrosine and serine phosphorylation. The amino acid sequences predicted in this study differ significantly from the amino acid sequence predicted previously for the Schmidt-Ruppin strain of Rous sarcoma virus.

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Base Sequence Chemical Phenomena Chemistry Chickens/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Viral Deoxyribonuclease EcoRI Genes, Viral Oncogene Protein pp60(v-src) Peptide Hydrolases/metabolism Phosphorylation Viral Envelope Proteins Viral Proteins/analysis,genetics
Chemicals
DNA, Viral Viral Envelope Proteins Viral Proteins Oncogene Protein pp60(v-src) DNA Restriction Enzymes Deoxyribonuclease EcoRI Peptide Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takeya T
Feldman R A
Hanafusa H
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42 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.1.1-11.1982
Published
1982-10-00
Pages
1-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256235
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · CA18213 · United States
Databases
GENBANK
K00928
External Links
PubMed source DOI: 10.1128/JVI.44.1.1-11.1982 PMC Full Text View journal details
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